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BAALC Polyclonal antibody, PBS Only (24997-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis of HEK-293 using 24997-1-AP (same clone as 24997-1-PBS)
HEK-293 cells were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.
WB analysis of A375 using 24997-1-AP (same clone as 24997-1-PBS)
A375 cells were subjected to SDS PAGE followed by western blot with 24997-1-AP (BAALC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)
IP result of anti-BAALC (IP:24997-1-AP, 4ug; Detection:24997-1-AP 1:300) with mouse brain tissue lysate 3000ug. This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24997-1-AP (BAALC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 24997-1-AP (same clone as 24997-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 24997-1-AP (BAALC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 24997-1-AP (same clone as 24997-1-PBS)
Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 24997-1-AP (BAALC antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 24997-1-PBS in a different storage buffer formulation.

BAALC Polyclonal antibody, PBS Only

Name: BAALC Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 24997-1-PBS

BAALC Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 24997-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

24997-1-PBS targets BAALC in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21850 Product name: Recombinant human BAALC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-145 aa of BC011517 Sequence: MGCGGSRADAIEPRYYESWTRETESTWLTYTDSDAPPSAAAPDSGPEAGGLHSGMLEDGLPSNGVPRSTAPGGIPNPEKKTNCETQCPNPQSLSSGPLTQKQNGLQTTEAKRDAKRMPAKEVTINVTDSIQQMDRSRRITKNCVN Predict reactive species
Full Name	brain and acute leukemia, cytoplasmic
Calculated Molecular Weight	19 kDa
Observed Molecular Weight	19-25 kDa
GenBank Accession Number	BC011517
Gene Symbol	BAALC
Gene ID (NCBI)	79870
RRID	AB_2879837
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8WXS3
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Brain and acute leukemia cytoplasmic protein, also named as BAALC, is a 180 amino acid protein, which interacts with CAMK2A. BAALC localizes in the cytoplasm and membrane. BAALC is expressed in neural and hematopoietic cells. Expression is found both in normal early progenitor cells and in the most immature type of blasts in acute leukemia but not in mature hematopoietic cells. BAALC may play a synaptic role at the postsynaptic lipid rafts by interacting with CAMK2A.