BACH1 Monoclonal antibody

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  WB analysis using 66762-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis of HEK-293 using 66762-1-Ig

  WB result of BACH1 antibody (66762-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BACH1 transfected HEK-293 cells.

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  WB analysis of HSC-T6 using 66762-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66762-1-Ig (BACH1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 66762-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 66762-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66762-1-Ig (BACH1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

BACH1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66762-1-Ig

Clone No.2D12A4

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF, ELISA

Synonyms

2D12A4, BACH 1, BACH-1

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, HSC-T6 cells, Jurkat cells, K-562 cells, Raji cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Product Information

66762-1-Ig targets BACH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BACH1 fusion protein Ag6011
• Full Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
• Calculated Molecular Weight: 82 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC063307
• Gene Symbol: BACH1
• Gene ID (NCBI): 571
• ENSEMBL Gene ID: ENSG00000156273
• RRID: AB_2882108
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O14867
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as a repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can function as transcriptional activators or repressors depending on the proteins with which they form heterodimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.

Publications

Species	Application	Title
mouse,human	WB,IF	Cell Rep; Targeting CXCR1 alleviates hyperoxia-induced lung injury through promoting glutamine metabolism; Authors - Hao Qin ; KD Validated
human	WB	Life Sci Alliance; A new ferrocene derivative blocks K-Ras localization and function by oxidative modification at His95; Authors - Kristen M Rehl
human	WB	Cancer Sci; BACH1 promotes clear cell renal cell carcinoma progression by upregulating oxidative stress-related tumorigenicity; Authors - Kenshiro Takemoto ; KD Validated