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BACH1 Monoclonal antibody
BACH1 Monoclonal Antibody for IHC, WB,ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Rat, mouse, pig, rabbit and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
2D12A4
Cat no : 66762-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | HeLa cells, rabbit brain tissue, HSC-T6 cells, HEK-293 cells, pig brain tissue, rat brain tissue, mouse brain tissue, Jurkat cells, K-562 cells, Raji cells |
Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
Product Information
66762-1-Ig targets BACH1 in WB, IHC, ELISA applications and shows reactivity with Human, Rat, mouse, pig, rabbit samples.
Tested Reactivity | Human, Rat, mouse, pig, rabbit |
Cited Reactivity | human |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | BACH1 fusion protein Ag6011 |
Full Name | BTB and CNC homology 1, basic leucine zipper transcription factor 1 |
Calculated molecular weight | 82 kDa |
Observed molecular weight | 100-110 kDa, 82 kDa |
GenBank accession number | BC063307 |
Gene symbol | BACH1 |
Gene ID (NCBI) | 571 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BTB and CNC homology 1, basic leucine zipper transcription factor 1(BACH1) is a heme-regulated transcriptional regulator that acts as repressor or activator and a member of the AP-a superfamily. MafK belongs to the small Maf family that can funciton as transcriptional activators or repressors depending on the proteins with which they form heterdimers. BACH1 has crucial roles in both stress responses and transformation by H-RasV12. The molecular weight of expected mobility Bach1 is 100-110 kDa.
Protocols
Product Specific Protocols | |
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WB protocol for BACH1 antibody 66762-1-Ig | Download protocol |
IHC protocol for BACH1 antibody 66762-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Cancer Sci BACH1 promotes clear cell renal cell carcinoma progression by upregulating oxidative stress-related tumorigenicity
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