BAG1 Polyclonal antibody

BAG1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 19064-1-AP

Synonyms

BAG 1, BAG1, BAG1L speicfic, BAG1M, BAG1S, HAP, RAP46



Tested Applications

Positive WB detected inHeLa cells, UV treated Hela , Jurkat cells, MCF-7 cells, HEK-293 cells, RAW264.7 , HT-1080 cells, HL-60 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman testis tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 19064-1-AP is BAG1 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BAG1 fusion protein Ag13555
Full Name BCL2-associated athanogene
Calculated molecular weight 39 kDa
Observed molecular weight 50-55 kDa, 30-33 kDa
GenBank accession numberBC014774
Gene symbol BAG1
Gene ID (NCBI) 573
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BAG1 have been identified that modulate gene transcription through poorly defined mechanisms. Four isoforms of the BAG1 protein (BAG1S, BAG1, BAG1M and BAG1L) can be produced from a common mRNA by use of alternative translation initiation sites, including a non-canonical CTG codon in one instance. The longest, BAG1L (Mr ~50K), contains a nuclear localization signal (NLS) and resides in the nucleus, whereas BAG1M (Mr ~46K) has an incomplete NLS and distributes mainly in cytosol, unless dragged into the nucleus through interactions with other. Distribution of BAG1S(p33) is not clear yet. This antibody can recognize all the isoforms of BAG1.

Protocols

Product Specific Protocols
WB protocol for BAG1 antibody 19064-1-APDownload protocol
IHC protocol for BAG1 antibody 19064-1-APDownload protocol
IF protocol for BAG1 antibody 19064-1-APDownload protocol
IP protocol for BAG1 antibody 19064-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Oncol Rep

Magnetic gold nanoparticle-mediated small interference RNA silencing Bag-1 gene for colon cancer therapy.

Authors - Wenbai Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Tongbin (Verified Customer) (08-25-2020)

This BAG1 antibody works very well in detecting endogenous BAG1 protein by western blots.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Heart