BAG5 Recombinant monoclonal antibody

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  WB analysis using 86248-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86248-1-RR (BAG5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  IF Staining of MCF-7 using 86248-1-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using BAG5 antibody (86248-1-RR, Clone: 250877D12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86248-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86248-1-RR against Human BAG5 were performed. The affinity constant is 0.286 nM.

BAG5 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86248-1-RR

Clone No.250877D12

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

BAG 5, BAG-5, Bcl-2-associated athanogene 5, KIAA0873

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, MCF-7 cells, A549 cells, mouse brain tissue, rat brain tissue, mouse lung tissue, rat lung tissue
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86248-1-RR targets BAG5 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag24320

  Product name: Recombinant human BAG5 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 46-394 aa of BC044216

  Sequence: NQHPSISRLQEIQKEVKSVEQQVIGFSGLSDDKNYKKLERILTKQLFEIDSVDTEGKGDIQQARKRAAQETERLLKELEQNANHPHRIEIQNIFEEAQSLVREKIVPFYNGGNCVTDEFEEGIQDIILRLTHVKTGGKISLRKARYHTLTKICAVQEIIEDCMKKQPSLPLSEDAHPSVAKINFVMCEVNKARGVLIALLMGVNNNETCRHLSCVLSGLIADLDALDVCGRTEIRNYRREVVEDINKLLKYLDLEEEADTTKAFDLRQNHSILKIEKVLKRMREIKNELLQAQNPSELYLSSKTELQGLIGQLDEVSLEKNPCIREARRRAVIEVQTLITYIDLKEALE

• Full Name: BCL2-associated athanogene 5
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48-51 kDa
• GenBank Accession Number: BC044216
• Gene Symbol: BAG5
• Gene ID (NCBI): 9529
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9UL15
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.