BBS10 Polyclonal antibody, PBS Only

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• BBS10 Polyclonal antibody, PBS Only (12421-2-PBS)

• 

  WB analysis of HepG2 using 12421-2-AP (same clone as 12421-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 12421-2-AP (same clone as 12421-2-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 12421-2-AP (same clone as 12421-2-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 12421-2-AP (BBS10 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 12421-2-AP (same clone as 12421-2-PBS)

  IF result (nuclear and the base of cilia stain) of anti-BBS10 (12421-2-AP; 1:50) with hTERT-RPE1 cell (PFA fixed) by Dr. Moshe Kim. This data was developed using the same antibody clone with 12421-2-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BBS10 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 12421-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

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Product Information

12421-2-PBS targets BBS10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3073

  Product name: Recombinant human BBS10 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 573-723 aa of BC013795

  Sequence: MLPVSCKLPNMGTSQSYLSSSMPAGCVLPVGGNFEILLHYYLLNYAKKCHQSEETMVSMIIANALLGIPKVLYKSKTGKYSFPHTYIRAVHALQTNQPLVSSQTGLESVMGKYQLLTSVLQCLTKILTIDMVITVKRHPQKVHNQDSEDEL

• Full Name: Bardet-Biedl syndrome 10
• Calculated Molecular Weight: 81 kDa
• Observed Molecular Weight: 81 kDa
• GenBank Accession Number: BC013795
• Gene Symbol: BBS10
• Gene ID (NCBI): 79738
• RRID: AB_2064962
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TAM1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BBS10, also named as C12orf58, belongs to the TCP-1 chaperonin family. It is probable molecular chaperone. Assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex, BBS10 may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Involved in adipogenic differentiation. (PMID:20080638,19190184) Defects in BBS10 are the cause of Bardet-Biedl syndrome type 10 (BBS10).