MKS1 Monoclonal antibody

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  WB analysis using 66518-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig brain using 66518-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 66518-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66518-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66518-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 66518-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MKS1 Monoclonal Antibody for WB, IHC, ELISA

Cat No. 66518-1-Ig

Clone No.6A8B11

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IHC, ELISA

Synonyms

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

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Tested Applications

• Positive WB detected in: NCCIT cells, pig brain tissue, HEK-293 cells, HSC-T6 cells
• Positive IHC detected in: mouse brain tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:150-1:600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66518-1-Ig targets MKS1 in WB, IHC, ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

• Tested Reactivity: Human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9504

  Product name: Recombinant human BBS13 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-321 aa of BC010061

  Sequence: MTTAASEVPSFLVERMANVRRRRQDRRGMEGGILKSRIVTWEPSEEFVRNNHVINTPLQTMHIMADLGPYKKLGYKKYEHVLCTLKVDSNGVITVKPDFTGLKGPYRIETEGEKQELWKYTIDNVSPHAQPEEEERERRVFKDLYGRHKEYLSSLVGTDFEMTVPGALRLFVNGEVVSAQGYEYDNLYVHFFVELPTAHWSSPAFQQLSGVTQTCTTKSLAMDKVAHFSYPFTFEAFFLHEDESSDALPEWPVLYCEVLSLDFWQRYRVEGYGAVVLPATPGSHTLTVSTWRPVELGTVAELRRFFIGGSLELEDLSYVRI

• Full Name: Meckel syndrome, type 1
• Calculated Molecular Weight: 559 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC010061
• Gene Symbol: MKS1
• Gene ID (NCBI): 54903
• RRID: AB_2881881
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9NXB0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.