MKS1 Monoclonal antibody, PBS Only

MKS1 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, pig, rat, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6A8B11

Cat no : 66518-1-PBS

Synonyms

BBS13, Meckel syndrome type 1 protein, Meckel syndrome, type 1, MES, MKS, MKS1

---

Validation Data Gallery

• 

  WB analysis using 66518-1-Ig (same clone as 66518-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66518-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig brain using 66518-1-Ig (same clone as 66518-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 66518-1-Ig (BBS13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66518-1-Ig (same clone as 66518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66518-1-Ig (same clone as 66518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 66518-1-Ig (same clone as 66518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66518-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66518-1-Ig (same clone as 66518-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66518-1-Ig (MKS1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66518-1-PBS in a different storage buffer formulation.

---

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66518-1-PBS targets MKS1 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, pig, rat, mouse samples.

• Tested Reactivity: Human, pig, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: MKS1 fusion protein Ag9504
• Full Name: Meckel syndrome, type 1
• Calculated Molecular Weight: 559 aa, 65 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC010061
• Gene Symbol: MKS1
• Gene ID (NCBI): 54903
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.