BBX Polyclonal antibody, PBS Only

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• BBX Polyclonal antibody, PBS Only (17254-1-PBS)

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  WB analysis of HepG2 using 17254-1-AP (same clone as 17254-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17254-1-AP (BBX antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

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  IP experiment of HepG2 using 17254-1-AP (same clone as 17254-1-PBS)

  IP result of anti-BBX (IP:17254-1-AP, 4ug; Detection:17254-1-AP 1:1000) with HepG2 cells lysate 2400ug. This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

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  IHC staining of human brain using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human brain using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human brain using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human spleen using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human spleen using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung using 17254-1-AP (same clone as 17254-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung using 17254-1-AP (BBX antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 17254-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

BBX Polyclonal Antibody for IHC, IP, Indirect ELISA

Cat No. 17254-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IP, Indirect ELISA

Synonyms

ARTC1, HBP2, HMG box-containing protein 2, HSPC339, MDS001

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Product Information

17254-1-PBS targets BBX in IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10691

  Product name: Recombinant human BBX protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 249-597 aa of BC012837

  Sequence: KPEDSDCHRKIETCGSRKSERSCKGALYKTLVSEGMLTSLRANVDRGKRSSGKGNSSDHEGCWNEESWTFSQSGTSGSKKFKKTKPKEDCLLGSAKLDEEFEKKFNSLPQYSPVTFDRKCVPVPRKKKKTGNVSSEPTKTSKGPFQSQKKNLFHKIVSKYKHKKEKPNVPEKGSGDKWSNKQLFLDAIHPTEAIFSEDRNTMEPVHKVKNIPSIFNTPEPTTTQEPLVGSQKRKARKTKITHLVRTADGRVSPAGGTLDDKPKEQLQRSLPKATETDCNDKCSHNTEVGETRSSTPEMPAVSAFFSLAALAEVAAMENVHRGQRSTPLTHDGQPKEMPQAPVLISCADQ

• Full Name: bobby sox homolog (Drosophila)
• Calculated Molecular Weight: 941 aa, 105 kDa
• Observed Molecular Weight: 105 kDa
• GenBank Accession Number: BC012837
• Gene Symbol: BBX
• Gene ID (NCBI): 56987
• RRID: AB_2065285
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WY36
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BBX, also named HBP2, is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As a DNA-binding domain in HBP2, HMG is essential for its function in Schizosaccharomyces pombe. HBP2 activates CdcIO-dependent transcription at G1/s and allows growth of cdc10-129, resldelta and rep2delta mutant cells at the restrictive temperature