BCAS3 Monoclonal antibody

BCAS3 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G7B1

Cat no : 67085-1-Ig

Synonyms

BCAS3, DKFZp686O1527, FLJ20128, GAOB1, MAAB, Protein Maab1

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Validation Data Gallery

• 

  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 67085-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67085-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67085-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67085-1-Ig (BCAS3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: U2OS cells, K-562 cells, Jurkat cells, A431 cells, HEK-293 cells, A549 cells, LNCaP cells, pig brain tissue, rat brain tissue, mouse rat tissue

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67085-1-Ig targets BCAS3 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BCAS3 fusion protein Ag28545
• Full Name: breast carcinoma amplified sequence 3
• Calculated Molecular Weight: 99 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC001250
• Gene Symbol: BCAS3
• Gene ID (NCBI): 54828
• RRID: AB_2882393
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Rudhira/BCAS3 (Breast Cancer Amplified Sequence 3) is a conserved protein expressed in the embryonic vasculature and malignant tumors. Rudhira is a predicted WD40 domain protein. During mouse development Rudhira is expressed in erythropoietic and angiogenic precursors but not in their differentiated progeny. This transient expression of Rudhira is also seen in normal adult angiogenesis. BCAS3, the human ortholog of Rudhira is 98% identical and the gene maps to a breakpoint of hematological neoplasms on chromosome 17q23.1. BCAS3 is also expressed in vascular development in embryoid bodies in vitro and was originally identified as a gene over-expressed in breast cancers. It is reported to be a target of metastasis associated protein-1 (MTA-1). Further BCAS3 expression is also upregulated in grade III and IV glioblastomas where it is misexpressed in the tumor cells.