CoraLite® Plus 488-conjugated BCAT1 Monoclonal antibody

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  WB analysis using CL488-67084

  Various lysates were subjected to SDS PAGE followed by western blot with CL488-67084 (BCAT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

BCAT1 Monoclonal Antibody for WB

Cat No. CL488-67084

Clone No.1G3A10

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB

Synonyms

MECA39, ECA39, EC:2.6.1.42, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT1/ECA39

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Tested Applications

• Positive WB detected in: Daudi cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67084 targets BCAT1 in WB applications and shows reactivity with human, pig samples.

• Tested Reactivity: human, pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag28328

  Product name: Recombinant human BCAT1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-320 aa of BC033864

  Sequence: MDCSNGCSAECTGEGGSKEVVGTFKAKDLIVTPATILKEKPDPNNLVFGTVFTDHMLTVEWSSEFGWEKPHIKPLQNLSLHPGSSALHYAVELFEGLKAFRGVDNKIRLFQPNLNMDRMYRSAVRATLPVFDKEELLECIQQLVKLDQEWVPYSTSASLYIRPTFIGTEPSLGVKKPTKALLFVLLSPVGPYFSSGTFNPVSLWANPKYVRAWKGGTGDCKMGGNYGSSLFAQCEAVDNGCQQVLWLYGEDHQITEVGTMNLFLYWINEDGEEELATPPLDGIILPGVTRRCILDLAHQWDTELSLFSINLPDFLQFIYF

• Full Name: branched chain aminotransferase 1, cytosolic
• Calculated Molecular Weight: 320 aa, 36 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC033864
• Gene Symbol: BCAT1
• Gene ID (NCBI): 586
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P54687
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BCAT1, also named as branched-chain amino acid trasaminase1, is a cytosolic enzyme responsible for the reversible transamination of leucine, isoleucine and valine that catalyzes the transformation of branched-chain L-amino acids (BCAA) into branched-chain a-ketoacids (BCKA), thereby providing macromolecule precursors. It has been reported that BCAT1 is associated with tumor growth and disease progression (PMID:29255149).