BCDIN3D Recombinant antibody

• 

  WB analysis using 83309-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83309-1-RR (BCDIN3D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HeLa using 83309-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug BCDIN3D Recombinant antibody (83309-1-RR, Clone:240152A9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  Affinity and Kinetic Characterization of 83309-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83309-1-RR against Human BCDIN3D were performed. The affinity constant is 2.38 nM.

BCDIN3D Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83309-1-RR

Clone No.240152A9

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

RNA 5'-monophosphate methyltransferase, EC:2.1.1.-, BCDIN3 domain-containing protein, 240152A9

---

Tested Applications

• Positive WB detected in: HeLa cells, SH-SY5Y cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83309-1-RR targets BCDIN3D in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BCDIN3D fusion protein Ag15613
• Full Name: BCDIN3 domain containing
• Calculated Molecular Weight: 292 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC053560
• Gene Symbol: BCDIN3D
• Gene ID (NCBI): 144233
• RRID: AB_3670975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z5W3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCDIN3D (BCDIN3 domain containing RNA methyltransferase), it is expected to be located in cytoplasm, which is ubiquitous expressed in prostate, lymph node and skeletal muscle. This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. The molecular weight of BCDIN3D is 33 kDa.