- Featured Product
- KD/KO Validated
CoraLite® Plus 647-conjugated BCL2 Polyclonal antibody
BCL2 Polyclonal Antibody for FC (Intra)
Host / Isotype
Rabbit / IgG
CoraLite® Plus 647 Fluorescent Dye
Cat no : CL647-12789
|Positive FC detected in||HeLa cells|
|Sample-dependent, check data in validation data gallery|
CL647-12789 targets BCL2 in FC (Intra) applications and shows reactivity with human samples.
|Host / Isotype||Rabbit / IgG|
|Immunogen||BCL2 fusion protein Ag3508|
|Full Name||B-cell CLL/lymphoma 2|
|Calculated molecular weight||26 kDa|
|Observed molecular weight||25-30 kDa|
|GenBank accession number||BC027258|
|Gene ID (NCBI)||596|
|Conjugate||CoraLite® Plus 647 Fluorescent Dye|
|Excitation/Emission maxima wavelengths||654 nm / 674 nm|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.|
|Storage Conditions||Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
1. What is Bcl-2?
Bcl-2 (B-cell lymphoma 2) is an outer mitochondrial membrane protein that via heterodimerization with BAX proteins controls apoptosis. Abnormalities of Bcl-2 activity can lead to cancer, autoimmune diseases, and schizophrenia.
2. FAQs for Bcl-2
a. How to measure Bcl-2 and Bax apoptotic markers by western blotting
Generally, Bcl-2 protein is considered to have anti-apoptotic activity, while Bax has apoptotic activity. Comparing the ratio between Bcl-2 and Bax protein levels in different samples or cells subjected to treatments can reflect the apoptotic state of the cells. Expression of Bax can vary between cell lines and it is important to compare it to Bcl-2 levels.
b. What band size should I expect in western blotting for Bcl-2?
The molecular weight of Bcl-2 is 26 kDa.
|Product Specific Protocols|
|FC protocol for CL Plus 647 BCL2 antibody CL647-12789||Download protocol|
|Click here to view our Standard Protocols|