Tested Applications
| Positive WB detected in | HeLa cells, mouse heart tissue, HepG2 cells, rat heart tissue |
| Positive IP detected in | mouse heart tissue |
| Positive IHC detected in | human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16612-1-AP targets BCL2L13 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9841 Product name: Recombinant human BCL2L13 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC003032 Sequence: MLLELTRRGQEPLSALLQFGVTYLEDYSAEYIIQQGGWGTVFSLESEEEEYPGITAEDSNDIYILPSDNSGQVSPPESPTVTTSWQSESLPVSLSASQSWHTESLPVSLGPESWQQIAMDPEEVKSLDSNGAGEKSENNSSNSDIVHVEKEEVPEGMEEAAVASVVLPARELQEALPEAPAPLLPHITATSLLGTREPDTEVITVEKSSPATSLFVELDEEEVKAATTEPTEVEEVVPALEPTETLLSEKEINAREESLVEELSPASEKKPVPPSEGKSRLSPAGEMKPMPLSEGKSILL Predict reactive species |
| Full Name | BCL2-like 13 (apoptosis facilitator) |
| Calculated Molecular Weight | 485 aa, 53 kDa |
| Observed Molecular Weight | 85 kDa |
| GenBank Accession Number | BC003032 |
| Gene Symbol | BCL2L13 |
| Gene ID (NCBI) | 23786 |
| RRID | AB_1850928 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BXK5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BCL2L13 antibody 16612-1-AP | Download protocol |
| IP protocol for BCL2L13 antibody 16612-1-AP | Download protocol |
| WB protocol for BCL2L13 antibody 16612-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BCL2L13 Polyclonal antibody (Cat# 16612-1-AP) has 32 citations. They appear in journals including Autophagy, Nature Communications, Neuron, Science Advances, Cell Reports, Cell Death & Differentiation, Cell Death & Disease, PNAS, Journal of Cell Biology, FEBS Journal, Journal of Biological Chemistry, PLoS Genetics, and bioRxiv. Research fields center on mitophagy, autophagy, mitochondrial biology, cancer, and neurodegenerative disease.
| Species | Application | Title |
|---|---|---|
Cancer Commun (Lond) Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites | ||
Autophagy Oxidative stress-preconditioned exosomes target BMF to restore mitophagy for alleviating intervertebral disc degeneration. | ||
Autophagy The mitophagy receptor BNIP3 is critical for the regulation of metabolic homeostasis and mitochondrial function in the nucleus pulposus cells of the intervertebral disc | ||
Autophagy BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. | ||
Nat Commun Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation. |
Reviews
What customers say
The single review for this product reports a 5-star rating for Western Blot. The user found the antibody worked well at a 1:1000 dilution, using Huh7 cell lysate that had been immunoprecipitated for a different target protein. Overall, the feedback is positive, confirming reliable WB performance.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Melissa (Verified Customer) (07-28-2022) | Antibody worked well in western blot. Lysate used was from Huh7 cells IP-ed for a different target protein.
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