BCL2L13 Polyclonal antibody

• Featured Product
• KD/KO Validated

BCL2L13 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

20

Reviews (1)

Cat no : 16612-1-AP

Synonyms

BCL2L13, MIL1, Protein Mil1

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Validation Data Gallery

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  WB analysis using 16612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16612-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16612-1-AP (BCL2L13 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 16612-1-AP

  IP result of anti-BCL2L13 (IP:16612-1-AP, 3ug; Detection:16612-1-AP 1:100) with mouse heart tissue lysate 8000ug.

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  IHC staining of human pancreas cancer using 16612-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16612-1-AP (BCL2L13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 16612-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 16612-1-AP (BCL2L13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse heart tissue, HepG2 cells, rat heart tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 5 publications below
• IF: See 3 publications below

Product Information

16612-1-AP targets BCL2L13 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL2L13 fusion protein Ag9841
• Full Name: BCL2-like 13 (apoptosis facilitator)
• Calculated Molecular Weight: 485 aa, 53 kDa
• Observed Molecular Weight: 85 kDa
• GenBank Accession Number: BC003032
• Gene Symbol: BCL2L13
• Gene ID (NCBI): 23786
• RRID: AB_1850928
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL2L13/MIL1 gene encodes a mitochondrially-localized protein with conserved B-cell lymphoma 2 homology motifs. BLC2L13 gene is highest expressed in heart, placenta and pancreas. The encoded protein Bcl-rambo may promote the activation of caspase-3 and apoptosis. The Bcl-rambo cell death activity was induced by its membrane-anchored C-terminal domain and not by the Bcl-2 homology region (PMID: 11262395). Multivariate analysis demonstrated that high expression of BCL2L13 was an independent prognostic factor for in childhood acute lymphoblastic leukemia (PMID: 20109966).

Publications

Species	Application	Title
mouse	IHC	Cancer Commun (Lond); Glucose-mediated mitochondrial reprogramming by cholesterol export at TM4SF5-enriched mitochondria-lysosome contact sites; Authors - Ji Eon Kim
human	WB	Adv Sci (Weinh); Mitochondrial tRNAGlu 14693A>G Mutation, an "Enhancer" to the Phenotypic Expression of Leber's Hereditary Optic Neuropathy; Authors - Lihao Jin
human	WB	Nat Commun; Bcl-2-like protein 13 is a mammalian Atg32 homologue that mediates mitophagy and mitochondrial fragmentation.; Authors - Tomokazu Murakawa
human,mouse	WB	Autophagy; BNIP3L/NIX degradation leads to mitophagy deficiency in ischemic brains.; Authors - Xiaoli Wu
mouse	WB	Autophagy; BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation.; Authors - Simone C da Silva Rosa
human	WB,IHC,IF	Proc Natl Acad Sci U S A; Bcl2L13 is a ceramide synthase inhibitor in glioblastoma.; Authors - Samuel A Jensen ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Melissa (Verified Customer) (07-28-2022)

Antibody worked well in western blot. Lysate used was from Huh7 cells IP-ed for a different target protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Huh7 cells

BCL2L13 Antibody Western Blot validation (1:1000 dilution) in Huh7 cells (Cat no:16612-1-AP)