BCL2L14 Polyclonal antibody

BCL2L14 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 12846-1-AP

Synonyms

Bcl2-L-14, Apoptosis regulator Bcl-G, Apoptosis regulator Bcl G, Apoptosis facilitator Bcl-2-like protein 14



Tested Applications

Positive WB detected inA549 cells, human lung tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12846-1-AP targets BCL2L14 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL2L14 fusion protein Ag3926
Full Name BCL2-like 14 (apoptosis facilitator)
Calculated Molecular Weight 327 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC025778
Gene Symbol BCL2L14
Gene ID (NCBI) 79370
RRIDAB_2290479
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BCL2L14 antibody 12846-1-APDownload protocol
IHC protocol for BCL2L14 antibody 12846-1-APDownload protocol
IF protocol for BCL2L14 antibody 12846-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols