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BCL2L15 Polyclonal antibody, PBS Only (23975-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis of SW480 using 23975-1-AP (same clone as 23975-1-PBS)
WB result of BCL2L15 antibody (23975-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BCL2L15 transfected SW480 cells. This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 23975-1-AP (same clone as 23975-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 23975-1-AP (same clone as 23975-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.
IHC staining of human colon cancer using 23975-1-AP (same clone as 23975-1-PBS)
Immunohistochemical analysis of paraffin-embedded colon cancer slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 23975-1-AP (same clone as 23975-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 23975-1-AP (BCL2L15 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 23975-1-AP (same clone as 23975-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.
IF Staining of HEK-293 using 23975-1-AP (same clone as 23975-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BCL2L15 antibody (23975-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23975-1-PBS in a different storage buffer formulation.

BCL2L15 Polyclonal antibody, PBS Only

Name: BCL2L15 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 23975-1-PBS

BCL2L15 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 23975-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

Bcl-2 family kin, Bcl2-L-15, Bcl-2-like protein 15, Bfk, C1orf178

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

23975-1-PBS targets BCL2L15 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag21155 Product name: Recombinant human BCL2L15 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-163 aa of BC127719 Sequence: MKSSQTFEEQTECIVNTLLMDFLSPTLQVASRNLCCVDEVDSGEPCSFDVAIIAGRLRMLGDQFNGELEASAKNVIAETIKGQTGAILQNTVESLSKTWCAQDSSLAYERAFLAVSVKLLEYMAHIAPEVVGQVAIPMTGMINGNQAIREFIQGQGGWENLES Predict reactive species
Full Name	BCL2-like 15
Calculated Molecular Weight	163 aa, 18 kDa
GenBank Accession Number	BC127719
Gene Symbol	BCL2L15
Gene ID (NCBI)	440603
RRID	AB_3085718
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q5TBC7
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

B-cell lymphoma 2 (Bcl-2)-like protein 15 (BCL2L15), also known as BCL2 family kin (BFK), belongs to the BCL2 protein family, and appears to induce weak apoptosis and antagonize the pro-survival function of Bcl2 (PMID:32708974). In addition, studies reported BCL2L15 expression in the stomach, ovaries, bone marrow, spleen, and uterus (PMID:12700646).