BCL9 Polyclonal antibody

BCL9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 22947-1-AP

Synonyms

Bcl-9, Protein legless homolog, Bcl 9, B-cell lymphoma 9 protein, B-cell CLL/lymphoma 9 protein

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Validation Data Gallery

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  WB analysis of Jurkat using 22947-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22947-1-AP (BCL9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 22947-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22947-1-AP (BCL9 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22947-1-AP (BCL9 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22947-1-AP (BCL9 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22947-1-AP (BCL9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 22947-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22947-1-AP (BCL9 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 22947-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using BCL9 antibody (22947-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: COLO 320 cells, Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells
• Positive IHC detected in: human liver cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

22947-1-AP targets BCL9 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BCL9 fusion protein Ag19158
• Full Name: B-cell CLL/lymphoma 9
• Calculated Molecular Weight: 1426 aa, 149 kDa
• Observed Molecular Weight: 149 kDa
• GenBank Accession Number: BC116451
• Gene Symbol: BCL9
• Gene ID (NCBI): 607
• RRID: AB_2879189
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Publications

Species	Application	Title
human	WB	Bioorg Chem; Discovery of 2-(isoxazol-5-yl)phenyl 3,4-dihydroxybenzoate as a potential inhibitor for the Wnt/β-catenin pathway; Authors - Yongxi Dong
human,mouse	WB,IHC	Reprod Biol Endocrinol; Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.; Authors - Xi Wang
human	WB,IF,IP	J Biol Chem; USP9X-mediated deubiquitination of B cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.; Authors - Zesen Shang