BCL9 Polyclonal antibody

BCL9 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 22947-1-AP

Synonyms

B cell CLL/lymphoma 9, B cell CLL/lymphoma 9 protein, B cell lymphoma 9 protein, BCL9, LGS, Protein legless homolog



Tested Applications

Positive WB detected inCOLO 320 cells, Jurkat cells, HEK-293 cells, HeLa cells, MCF-7 cells
Positive IHC detected inhuman liver cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22947-1-AP targets BCL9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BCL9 fusion protein Ag19158
Full Name B-cell CLL/lymphoma 9
Calculated Molecular Weight 1426 aa, 149 kDa
Observed Molecular Weight 149 kDa
GenBank Accession NumberBC116451
Gene Symbol BCL9
Gene ID (NCBI) 607
RRIDAB_2879189
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BCL9 is associated with B-cell acute lymphoblastic leukemia. It may be a target of translocation in B-cell malignancies with abnormalities of 1q21. Its function is unknown. The overexpression of BCL9 may be of pathogenic significance in B-cell malignancies.

Protocols

Product Specific Protocols
WB protocol for BCL9 antibody 22947-1-APDownload protocol
IHC protocol for BCL9 antibody 22947-1-APDownload protocol
IF protocol for BCL9 antibody 22947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Bioorg Chem

Discovery of 2-(isoxazol-5-yl)phenyl 3,4-dihydroxybenzoate as a potential inhibitor for the Wnt/β-catenin pathway

Authors - Yongxi Dong
human,mouseWB,IHC

Reprod Biol Endocrinol

Exosomal AFAP1-AS1 binds to microRNA-15a-5p to promote the proliferation, migration, and invasion of ectopic endometrial stromal cells in endometriosis.

Authors - Xi Wang
humanWB,IF,IP

J Biol Chem

USP9X-mediated deubiquitination of B cell CLL/lymphoma 9 potentiates Wnt signaling and promotes breast carcinogenesis.

Authors - Zesen Shang