BCLAF1 Monoclonal antibody

BCLAF1 Monoclonal Antibody for IF, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IF,ELISA

Conjugate

Unconjugated

CloneNo.

1D6G7

Cat no : 67860-1-Ig

Synonyms

BCLAF1, bK211L9.1, BTF, KIAA0164



Tested Applications

Positive WB detected inLNCaP cells, K-562 cells, HeLa cells, Jurkat cells, HEK-293 cells
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:400-1:1600
Sample-dependent, check data in validation data gallery

Product Information

67860-1-Ig targets BCLAF1 in WB, IF,ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen BCLAF1 fusion protein Ag25210
Full Name BCL2-associated transcription factor 1
Calculated molecular weight 920 aa, 106 kDa
Observed molecular weight145 kDa
GenBank accession numberBC132780
Gene symbol BCLAF1
Gene ID (NCBI) 9774
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

BCLF1, also named as Bcl-2-associated transcription factor 1, is a 920 amino acid protein, which Interacts with Bcl-2 related proteins, EMD, with the adenovirus E1B 19 kDa protein and with DNA. BCLF1 as a death-promoting transcriptional repressor may be involved in cyclin-D1/CCND1 mRNA stability through the SNARP complex which associates with both the 3'end of the CCND1 gene and its mRNA. The calculated molecular weight of BCLF1 is a 110 kDa, but the modified BCLF1 protein is about 140-150 kDa.

Protocols

Product Specific Protocols
WB protocol for BCLAF1 antibody 67860-1-IgDownload protocol
IF protocol for BCLAF1 antibody 67860-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols