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BCORL1 Polyclonal antibody, PBS Only (24315-1-PBS)

Filter: AllWBIHCIF/ICC

WB analysis using 24315-1-AP (same clone as 24315-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.
WB analysis using 24315-1-AP (same clone as 24315-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 24315-1-AP (BCORL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.
IHC staining of mouse colon using 24315-1-AP (same clone as 24315-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 24315-1-AP (same clone as 24315-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 24315-1-AP (same clone as 24315-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24315-1-AP (BCORL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.
IF Staining of U2OS using 24315-1-AP (same clone as 24315-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed U2OS cells using BCORL1 antibody (24315-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 24315-1-PBS in a different storage buffer formulation.

BCORL1 Polyclonal antibody, PBS Only

Name: BCORL1 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 24315-1-PBS

BCORL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24315-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

24315-1-PBS targets BCORL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag19522 Product name: Recombinant human BCORL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC156742 Sequence: MISTAPLYSGVHNWTSSDRIRMCGINEERRAPLSDEESTTGDCQHFGSQEFCVSSSFSKVELTAVGSGSNARGADPDGSATEKLGHKSEDKPDDPQPKMDYAGNVAEAEGFLVPLSSPGDGLKLPASDSAEASNSRADCSWTPLNTQMSKQVDCSPAGVKALDSRQGVGEKNTFILATLGTGVPVEGTLPLVTTNFSPLPAPICPPAPGSASVPHSVPDAFQVPLSVPAPVPHSGLVPVQVATSVPAPSPPLAPVPALAPAPPSVPTLISDSNPLSVSASVLVPVPASAPPSGPVPLSAPAPAPLSVPVSAPPLALIQAPVPPSAPTLVLAPVPTPVLAPMPASTPPAA Predict reactive species
Full Name	BCL6 co-repressor-like 1
Calculated Molecular Weight	1711 aa, 183 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	BC156742
Gene Symbol	BCORL1
Gene ID (NCBI)	63035
RRID	AB_3669432
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5H9F3
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

BCORL1 is a transcriptional corepressor found tethered to promoter regions by DNA-binding proteins. BCORL1 can interact with class II histone deacetylases, and interact with the CtBP corepressor through the CtBP binding motif-PXDLS (PMID: 21989985, 17379597). The open reading frame of BCORL1 cDNA encodes a protein of 1711 amino acids. The antibody recognized a band migrating through SDS-polyacrylamide gels at a size of ∼200 kDa.