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- KD/KO Validated
BDH2 Polyclonal antibody
BDH2 Polyclonal Antibody for IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
Cat no : 27207-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | BxPC-3 cells, HeLa cells |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 1 publications below |
IHC | See 2 publications below |
Product Information
The immunogen of 27207-1-AP is BDH2 Fusion Protein expressed in E. coli.
Tested Reactivity | human |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | BDH2 fusion protein Ag24934 |
Full Name | 3-hydroxybutyrate dehydrogenase, type 2 |
Observed molecular weight | 27 kDa |
GenBank accession number | BC095414 |
Gene symbol | BDH2 |
Gene ID (NCBI) | 56898 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
Product Specific Protocols | |
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WB protocol for BDH2 antibody 27207-1-AP | Download protocol |
IHC protocol for BDH2 antibody 27207-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer. | ||
J Hematol Oncol Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.
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