BDH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BDH2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 27207-1-AP

Synonyms

BDH2, DHRS6, EFA6R, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308

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Validation Data Gallery

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  WB analysis of BxPC-3 using 27207-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below

Product Information

The immunogen of 27207-1-AP is BDH2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BDH2 fusion protein Ag24934
• Full Name: 3-hydroxybutyrate dehydrogenase, type 2
• Observed molecular weight: 27 kDa
• GenBank accession number: BC095414
• Gene symbol: BDH2
• Gene ID (NCBI): 56898
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Publications

Species	Application	Title
human	IHC	J Exp Clin Cancer Res; BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer.; Authors - Jia-Zhou Liu
human,mouse	WB,IHC	J Hematol Oncol; Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.; Authors - Tianxiao Yang ; KD Validated