BDH2 Polyclonal antibody

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  WB analysis of HeLa using 27207-1-AP

  WB result of BDH2 antibody (27207-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

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  WB analysis using 27207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27207-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

BDH2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27207-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

(R)-beta-hydroxybutyrate dehydrogenase, 3-hydroxybutyrate dehydrogenase type 2, 4-oxo-L-proline reductase, DHRS6, EC:1.1.1.-

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below

Product Information

27207-1-AP targets BDH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag24934

  Product name: Recombinant human BDH2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-245 aa of BC095414

  Sequence: MGRLDGKVIILTAAAQGIGQAAALAFAREGAKVIATDINESKLQELEKYPGIQTRVLDVTKKKQIDQFANEVERLDVLFNVAGFVHHGTVLDCEEKDWDFSMNLNVRSMYLMIKAFLPKMLAQKSGNIINMSSVASSVKGVVNRCVYSTTKAAVIGLTKSVAADFIQQGIRCNCVCPGTVDTPSLQERIQARGNPEEARNDFLKRQKTGRFATAEEIAMLCVYLASDESTYVTGNPVIIDGGWSL

• Full Name: 3-hydroxybutyrate dehydrogenase, type 2
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC095414
• Gene Symbol: BDH2
• Gene ID (NCBI): 56898
• RRID: AB_2880800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BUT1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type 2-hydroxybutyrate dehydrogenase (BDH2) is a short-chain dehydrogenase/reductase family member involved in several biological and pathological processes, including the utilization of cytosolic ketone bodies, immunocyte regulation and tumor progression. As a member of a gene family involved in ketone metabolism, BDH2 upregulated the level of β-HB in liver cells as well as the level of H3 histone acetylation (PMID: 25762501). Downregulation of BDH2 during infection is due to activation of hepcidin and intracellular iron retention, indicating an important role for BDH2 in iron-limiting innate immunity and host defense (PMID: 31333791, 32344823).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	J Clin Invest; Iron-dependent epigenetic modulation promotes pathogenic T cell differentiation in lupus.; Authors - Xiaofei Gao
mouse,human	WB,IHC	J Hematol Oncol; Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.; Authors - Tianxiao Yang ; KD Validated
human	IHC	J Exp Clin Cancer Res; BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer.; Authors - Jia-Zhou Liu
mouse	WB	J Proteome Res; 3-Hydroxybutyrate Promotes Myoblast Proliferation and Differentiation through Energy Metabolism and GPR109a-Mediated Ca2+-NFAT Signaling Pathways; Authors - Xu Qiu
human	WB	Nat Metab; BDH2-driven lysosome-to-mitochondria iron transfer shapes ferroptosis vulnerability of the melanoma cell states; Authors - Francesca Rizzollo ; KD Validated