BDH2 Polyclonal antibody

• Featured Product
• KD/KO Validated

BDH2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 27207-1-AP

Synonyms

BDH2, DHRS6, EFA6R, Oxidoreductase UCPA, PRO20933, SDR15C1, UCPA OR, UNQ6308

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Validation Data Gallery

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  WB analysis of HeLa using 27207-1-AP

  WB result of BDH2 antibody (27207-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-BDH2 transfected HeLa cells.

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  WB analysis using 27207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 27207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27207-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27207-1-AP (BDH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 27207-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27207-1-AP (BDH2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below

Product Information

27207-1-AP targets BDH2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BDH2 fusion protein Ag24934
• Full Name: 3-hydroxybutyrate dehydrogenase, type 2
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC095414
• Gene Symbol: BDH2
• Gene ID (NCBI): 56898
• RRID: AB_2880800
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	J Clin Invest; Iron-dependent epigenetic modulation promotes pathogenic T cell differentiation in lupus.; Authors - Xiaofei Gao
human,mouse	WB,IHC	J Hematol Oncol; Increased B3GALNT2 in hepatocellular carcinoma promotes macrophage recruitment via reducing acetoacetate secretion and elevating MIF activity.; Authors - Tianxiao Yang ; KD Validated
human	IHC	J Exp Clin Cancer Res; BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer.; Authors - Jia-Zhou Liu