Osteocalcin Polyclonal antibody

Osteocalcin Polyclonal Antibody for FC, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 16157-1-AP

Synonyms

BGLAP, BGP, Bone Gla protein, OC, OCN, Osteocalcin, PMF1

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Validation Data Gallery

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of NIH/3T3 using 16157-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin antibody (16157-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of U2OS using 16157-1-AP

  1X10^6 U2OS cells were intracellularly stained with 0.4 ug Anti-Human Osteocalcin (16157-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IHC detected in: human osteosarcoma tissue, mouse lung tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: NIH/3T3 cells
• Positive FC detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16157-1-AP targets Osteocalcin in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: bone gamma-carboxyglutamate (gla) protein
• Calculated molecular weight: 11 kDa
• GenBank accession number: NM_199173
• Gene symbol: BGLAP
• Gene ID (NCBI): 632
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Aging (Albany NY); Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.; Authors - Jie Xu
mouse	IHC	Front Pharmacol; Naringenin is a Potential Anabolic Treatment for Bone Loss by Modulating Osteogenesis, Osteoclastogenesis, and Macrophage Polarization.; Authors - Xin Zhou

Related Products

Flow Cytometry Perm Buffer (10X)

PF00011-C