Osteocalcin/OCN Polyclonal antibody

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human osteosarcoma using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded human osteosarcoma tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 16157-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 16157-1-AP (Osteocalcin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of NIH/3T3 using 16157-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using Osteocalcin/OCN antibody (16157-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  FC experiment of U2OS using 16157-1-AP

  1x10^6 U2OS cells were intracellularly stained with 0.4 ug Osteocalcin/OCN Polyclonal antibody (16157-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Osteocalcin/OCN Polyclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Cat No. 16157-1-AP

Publications(10)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

BGLAP, Osteocalcin, BGP, Bone Gla protein, Gamma-carboxyglutamic acid-containing protein

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Tested Applications

• Positive IHC detected in: human osteosarcoma tissue, mouse lung tissue, rat lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: NIH/3T3 cells
• Positive FC (Intra) detected in: U2OS cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Product Information

16157-1-AP targets Osteocalcin/OCN in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: bone gamma-carboxyglutamate (gla) protein
• Calculated Molecular Weight: 11 kDa
• GenBank Accession Number: NM_199173
• Gene Symbol: Osteocalcin
• Gene ID (NCBI): 632
• RRID: AB_2878225
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P02818
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	IF	ACS Appl Mater Interfaces; Cerium-Organic Framework and Resveratrol Composite Hydrogel Scaffold with Dual Antioxidant Activity for Enhanced Bone Regeneration; Authors - Yao Lu
mouse	IF	Aging (Albany NY); Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway.; Authors - Jie Xu
mouse	IHC	Front Pharmacol; Naringenin is a Potential Anabolic Treatment for Bone Loss by Modulating Osteogenesis, Osteoclastogenesis, and Macrophage Polarization.; Authors - Xin Zhou
mouse	IHC	Bone; Macrophage migration inhibitory factor promotes heterotopic ossification by mediating ROS/HIF-1α positive feedback loop and activating Wnt/β-catenin signaling pathway; Authors - Ping Li
mouse	WB	J Cell Mol Med; KIAA0753 enhances osteoblast differentiation suppressed by diabetes; Authors - Mengxue Li
rat	IF	Sci Rep; Ginsenoside Rg1 regulating inflammatory response and bone-remodeling through Keap1/Nrf2 signaling pathway in rats with periodontitis; Authors - Yang Zhou