Bim Polyclonal antibody

Bim Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22037-1-AP

Synonyms

BAM, BCL2L11, BIM, BIM alpha6, BIM beta6, BIM beta7, BimEL, BimL, BOD



Tested Applications

Positive WB detected inRAW 264.7 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22037-1-AP targets Bim in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bim fusion protein Ag11875
Full Name BCL2-like 11 (apoptosis facilitator)
Calculated Molecular Weight 198 aa, 22 kDa
Observed Molecular Weight 23 and 18 kDa
GenBank Accession NumberBC033694
Gene Symbol Bim
Gene ID (NCBI) 10018
RRIDAB_2878978
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Bim antibody 22037-1-APDownload protocol
IHC protocol for Bim antibody 22037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Filamentous GLS1 promotes ROS-induced apoptosis upon glutamine deprivation via insufficient asparagine synthesis.

Authors - Bin Jiang
humanWB

Aging (Albany NY)

Extracorporeal shockwave relieves endothelial injury and dysfunction in steroid-induced osteonecrosis of the femoral head via miR-135b targeting FOXO1: in vitro and in vivo studies.

Authors - Xinjie Wu
humanWB

Virulence

Inhibition of cell proliferation by Tas of foamy viruses through cell cycle arrest or apoptosis underlines the different mechanisms of virus-host interactions.

Authors - Wei Jie
humanWB

Oxid Med Cell Longev

One-Two Punch Therapy for the Treatment of T-Cell Malignancies Involving p53-Dependent Cellular Senescence

Authors - Yingjie Qing
mouseWB

Biochem Pharmacol

Inhibition of IRE1 Suppresses the Catabolic Effect of IL-1β on Nucleus Pulposus Cell and Prevents Intervertebral Disc Degeneration in vivo.

Authors - Honglei Kang
mouseWB

Mol Cells

Sertad1 Induces Neurological Injury after Ischemic Stroke via the CDK4/p-Rb Pathway.

Authors - Jianxiong Li