Bim Polyclonal antibody

Bim Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22037-1-AP

Synonyms

BAM, BCL2L11, BIM, BIM alpha6, BIM beta6, BIM beta7, BimEL, BimL, BOD



Tested Applications

Positive WB detected inRAW 264.7 cells, Raji cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22037-1-AP is Bim Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited ReactivityHUMAN
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Bim fusion protein Ag11875
Full Name BCL2-like 11 (apoptosis facilitator)
Calculated molecular weight 198 aa, 22 kDa
Observed molecular weight 23 and 18 kDa
GenBank accession numberBC033694
Gene symbol BCL2L11
Gene ID (NCBI) 10018
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for Bim antibody 22037-1-APDownload protocol
IHC protocol for Bim antibody 22037-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC, WB

Mol Med Rep

Inhibition of Bcl-2 and Bcl-xL overcomes the resistance to the third-generation EGFR tyrosine kinase inhibitor osimertinib in non-small cell lung cancer.

Authors - Yingjie Lu
HUMANWB

Onco Targets Ther

Inhibition of FOXO1 by small interfering RNA enhances proliferation and inhibits apoptosis of papillary thyroid carcinoma cells via Akt/FOXO1/Bim pathway.

Authors - Hong-Ming Song