BIN3 Polyclonal antibody

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  WB analysis of K-562 using 11215-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11215-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11215-1-AP (BIN3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 11215-1-AP

  IP result of anti-BIN3 (IP:11215-1-AP, 4ug; Detection:11215-1-AP 1:300) with K-562 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 11215-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11215-1-AP (BIN3 antibody) at dilution of 1:50 (under 10x lens).

BIN3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11215-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

BIN3, bridging integrator 3

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

11215-1-AP targets BIN3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag1713

  Product name: Recombinant human BIN3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-253 aa of BC009824

  Sequence: MSWIPFKIGQPKKQIVPKTVERDFEREYGKLQQLEEQTRRLQKDMKKSTDADLAMSKSAVKISLDLLSNPLCEQDQDLLNMVTALDTAMKRMDAFNQEKVNQIQKTVIEPLKKFGSVFPSLNMAVKRREQALQDYRRLQAKVEKYEEKEKTGPVLAKLHQAREELRPVREDFEAKNRQLLEEMPRFYGSRLDYFQPSFESLIRAQVVYYSEMHKIFGDLSHQLDQPGHSDEQRERENEAKLSELRALSIVADD

• Full Name: bridging integrator 3
• Calculated Molecular Weight: 30 kDa
• Observed Molecular Weight: 30 kDa
• GenBank Accession Number: BC009824
• Gene Symbol: BIN3
• Gene ID (NCBI): 55909
• RRID: AB_2063649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9NQY0
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Bridging integrator 3 (BIN3) is a conserved BAR (Bin/amphiphysin/ Rvs) domain adaptor protein (PMID: 11274158). BAR domain proteins, sharing an N-terminal BAR domain, have been implicated in diverse cellular processes, including endocytosis, vesicle fusion, cell polarity, actin organization, signal transduction, transcription repression, and tumor suppression (PMID: 16524918). BIN3 is involved in cytokinesis and septation where it has a role in the localization of F-actin (PMID: 11274158).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Med; Implication of the miR-184 and miR-204 competitive RNA network in control of mouse secondary cataract.; Authors - Hoffmann Andrea A