BLZF1 Polyclonal antibody

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  WB analysis of K-562 using 16354-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 16354-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16354-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16354-1-AP (BLZF1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse liver using 16354-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16354-1-AP (BLZF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BLZF1 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16354-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

GOLGIN 45, Golgin-45, JEM 1, JEM1, JEM-1

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Tested Applications

• Positive WB detected in: K-562 cells, Jurkat cells
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

16354-1-AP targets BLZF1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9564

  Product name: Recombinant human BLZF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 48-400 aa of BC020716

  Sequence: QSPWKKAVPSESPGVLQLGKMLTEKAMEVKAVRILVPKAAITHDIPNKNTKVKSLGHHKGEFLGQSEGVIEPNKELSEVKNVLEKLKNSERRLLQDKEGLSNQLRVQTEVNRELKKLLVASVGDDLQYHFERLAREKNQLILENEALGRNTAQLSEQLERMSIQCDVWRSKFLASRVMADELTNSRAALQRQNRDAHGAIQDLLSEREQFRQEMIATQKLLEELLVSLQWGREQTYSPSVQPHSTAELALTNHKLAKAVNSHLLGNVGINNQKKIPSTVEFCSTPAEKMAETVLRILDPVTCKESSPDNPFFESSPTTLLATKKNIGRFHPYTRYENITFNCCNHCRGELIAL

• Full Name: basic leucine zipper nuclear factor 1
• Calculated Molecular Weight: 400 aa, 45 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC020716
• Gene Symbol: BLZF1
• Gene ID (NCBI): 8548
• RRID: AB_2918045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H2G9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BLZF1 (basic leucine zipper nuclear factor 1), also known as JEM1 or Golgin-45, maps to human chromosome 1q24, encodes for a 3 kb mRNA that is translated into a 45 kDa protein. BLZF1 is a member of b-ZIP family, and has been implicated in epigenetic regulation and Wnt/β-catenin signaling.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Biol Cell; Knockout of the Golgi stacking proteins GRASP55 and GRASP65 impairs Golgi structure and function.; Authors - Michael E Bekier
human	WB	Methods Mol Biol; Immunofluorescence Microscopy of the Mammalian Golgi Apparatus; Authors - Maryam Arab
	WB	Methods Mol Biol; Common Markers and Small Molecule Inhibitors in Golgi Studies; Authors - Sarah Bui