BNIP3 Monoclonal antibody, PBS Only

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  WB analysis of NIH/3T3 using 68091-1-Ig (same clone as 68091-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 68091-1-Ig (same clone as 68091-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 68091-1-Ig (same clone as 68091-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse kidney using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 68091-1-Ig (BNIP3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 68091-1-Ig (same clone as 68091-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68091-1-PBS in a different storage buffer formulation.

BNIP3 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 68091-1-PBS

Clone No.2H7D8

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

2H7D8, NIP3

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Product Information

68091-1-PBS targets BNIP3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27935

  Product name: Recombinant human BNIP3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-194 aa of BC009342

  Sequence: MQEESLQGSWVELHFSNNGNGGSVPASVSIYNGDMEKILLDAQHESGRSSSKSSHCDSPPRSQTPQDTNRASETDTHSIGEKNSSQSEEDDIERRKEVESILKKNSDWIWDWSSRPENIPPKEFLFKHPKRTATLSMRNTSVMKKGGIFSAEFLKVFLPSLLLSHLLAIGLGIYIGRRLTTSTSTF

• Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3
• Calculated Molecular Weight: 194 aa, 22 kDa
• Observed Molecular Weight: 25-30,50-60 kDa
• GenBank Accession Number: BC009342
• Gene Symbol: BNIP3
• Gene ID (NCBI): 664
• RRID: AB_2918828
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q12983
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.