Tested Applications
| Positive WB detected in | HepG2 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells |
| Positive IF/ICC detected in | Cobalt Chloride treated HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 25 publications below |
| IHC | See 6 publications below |
| IF | See 5 publications below |
| CoIP | See 1 publications below |
Product Information
68091-1-Ig targets BNIP3 in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with Human, Mouse, rat samples.
| Tested Reactivity | Human, Mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | BNIP3 fusion protein Ag27935 Predict reactive species |
| Full Name | BCL2/adenovirus E1B 19kDa interacting protein 3 |
| Calculated Molecular Weight | 194 aa, 22 kDa |
| Observed Molecular Weight | 25-30,50-60 kDa |
| GenBank Accession Number | BC009342 |
| Gene Symbol | BNIP3 |
| Gene ID (NCBI) | 664 |
| RRID | AB_2918828 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q12983 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BNIP3 antibody 68091-1-Ig | Download protocol |
| IF protocol for BNIP3 antibody 68091-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Ammonia-induced lysosomal and mitochondrial damage causes cell death of effector CD8+ T cells | ||
Small Honeycomb Bionic Graphene Oxide Quantum Dot/Layered Double Hydroxide Composite Nanocoating Promotes Osteoporotic Bone Regeneration via Activating Mitophagy | ||
Cell Biosci Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy | ||
Cell Rep Proline restores mitochondrial function and reverses aging hallmarks in senescent cells | ||
Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma | ||
Cell Commun Signal Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1 |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Paula (Verified Customer) (07-26-2024) | Worked well for Western Blotting
|
FH Aidan (Verified Customer) (04-10-2024) | worked v well, would highly recommend. Incubated overnight 4 degrees 1:5000.
 |
