BNIP3 Monoclonal antibody

BNIP3 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

5

CloneNo.

2H7D8

Cat no : 68091-1-Ig

Synonyms

BNIP3, NIP3

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 68091-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68091-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HSC-T6 using 68091-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 68091-1-Ig (BNIP3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 68091-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed Cobalt Chloride treated HepG2 cells using BNIP3 antibody (68091-1-Ig, Clone: 2H7D8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: HepG2 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells
• Positive IF detected in: Cobalt Chloride treated HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF): IF : 1:200-1:800

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below

Product Information

68091-1-Ig targets BNIP3 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: BNIP3 fusion protein Ag27935
• Full Name: BCL2/adenovirus E1B 19kDa interacting protein 3
• Calculated molecular weight: 194 aa, 22 kDa
• Observed molecular weight: 25-30,50-60 kDa
• GenBank accession number: BC009342
• Gene symbol: BNIP3
• Gene ID (NCBI): 664
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

Publications

Species	Application	Title
mouse	WB	Cell Biosci; Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy; Authors - Mei-Fei Wu
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
mouse	WB,IHC	Cell Commun Signal; Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1; Authors - Xudong Xie
human	WB	ACS Appl Mater Interfaces; Arsenic Prodrug-Mediated Tumor Microenvironment Modulation Platform for Synergetic Glioblastoma Therapy; Authors - Jiliang Yan
human	WB	Autophagy; Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1; Authors - Ying Liu