BNIP3 Monoclonal antibody

BNIP3 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2H7D8

Cat no : 68091-1-Ig

Synonyms

BNIP3, NIP3



Tested Applications

Positive WB detected inHepG2 cells, HSC-T6 cells, NIH/3T3 cells, LNCaP cells
Positive IF detected inCobalt Chloride treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

68091-1-Ig targets BNIP3 in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen BNIP3 fusion protein Ag27935
Full Name BCL2/adenovirus E1B 19kDa interacting protein 3
Calculated molecular weight 194 aa, 22 kDa
Observed molecular weight25-30,50-60 kDa
GenBank accession numberBC009342
Gene symbol BNIP3
Gene ID (NCBI) 664
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BNIP3 (BCL2 interacting protein 3) is a member of the Bcl-2 family that is expressed in mitochondria and induces apoptosis (PMID: 10891486). It can regulate the permeabillity state of outer mitchondrial member (OMM), this regulation is accompanied by the formation of homo- and hetero-oligomers inside the membrane. Hypoxia can lead to transcriptional activation of BNIP3 through HIF-1α (PMID: 11559532). It's calculated molecule weight is 21.5 kDa. The antibody can detect bands around 25 and 50 kDa, representing monomeric and dimeric forms, respectively (PMID: 21364626).BNIP3 is related to the occurrence and development of tumors, cardiovascular diseases, and Parkinson's diseases.

Protocols

Product Specific Protocols
WB protocol for BNIP3 antibody 68091-1-IgDownload protocol
IF protocol for BNIP3 antibody 68091-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Biosci

Hif-2α regulates lipid metabolism in alcoholic fatty liver disease through mitophagy

Authors - Mei-Fei Wu
humanWB

Front Cell Dev Biol

Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma

Authors - Wenshuo Zhang
mouseWB,IHC

Cell Commun Signal

Metformin alleviates bone loss in ovariectomized mice through inhibition of autophagy of osteoclast precursors mediated by E2F1

Authors - Xudong Xie
humanWB

ACS Appl Mater Interfaces

Arsenic Prodrug-Mediated Tumor Microenvironment Modulation Platform for Synergetic Glioblastoma Therapy

Authors - Jiliang Yan
humanWB

Autophagy

Hypoxia-induced GPCPD1 depalmitoylation triggers mitophagy via regulating PRKN-mediated ubiquitination of VDAC1

Authors - Ying Liu