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BOULE Polyclonal antibody, PBS Only (13720-1-PBS)

Filter: AllWBIPIHCIF-PIF-Fro

WB analysis using 13720-1-AP (same clone as 13720-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 13720-1-AP (BOULE antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IP experiment of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)
IP result of anti-BOULE (IP:13720-1-AP, 4ug; Detection:13720-1-AP 1:1000) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IHC staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IHC staining of mouse ovary using 13720-1-AP (same clone as 13720-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 13720-1-AP (BOULE antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using BOULE antibody (13720-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.
IF Staining of mouse testis using 13720-1-AP (same clone as 13720-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse testis tissue using 13720-1-AP (BOULE antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13720-1-PBS in a different storage buffer formulation.

BOULE Polyclonal antibody, PBS Only

Name: BOULE Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 13720-1-PBS

BOULE Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Cat No. 13720-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA

Synonyms

BOLL, Protein boule-like

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

13720-1-PBS targets BOULE in WB, IHC, IF-P, IF-Fro, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4662 Product name: Recombinant human BOLL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-283 aa of BC033674 Sequence: MQTDSLSPSPNPVSPVPLNNPTSAPRYGTVIPNRIFVGGIDFKTNESDLRKFFSQYGSVKEVKIVNDRAGVSKGYGFVTFETQEDAQKILQEAEKLNYKDKKLNIGPAIRKQQVGIPRSSIMPAAGTMYLTTSTGYPYTYHNGVAYFHTPEVTSVPPPWPSRSVCSSPVMVAQPIYQQPAYHYQATTQYLPGQWQWSVPQPSASSAPFLYLQPSEVIYQPVEIAQDGGCVPPPLSLMETSVPEPYSDHGVQATYHQVYAPSAITMPAPVMQPEPIKTVWSIHY Predict reactive species
Full Name	bol, boule-like (Drosophila)
Calculated Molecular Weight	283 aa, 31 kDa
Observed Molecular Weight	38-42 kDa
GenBank Accession Number	BC033674
Gene Symbol	BOULE
Gene ID (NCBI)	66037
RRID	AB_2290617
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8N9W6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

BOULE, also called BOLL, belongs to the RRM DAZ family. BOULE has been identified as being involved in regulating the development and differentiation of germ cells and playing an essential role in infertility (PMID: 31683986). BOULE is specifically expressed in testis, but not in early embryos, primordial germ cells, and spermatogonial cells. It is first expressed in the cytoplasm of spermatocytes and then persists through meiosis. BOULE has 5 isoforms with the molecular mass of 19 and 31-38 kDa. This antibody detects two bands in testis, the lower band of ~40 kDa in size corresponds to the predicted endogenous BOULE protein, while the upper band may be an isoform or posttranslationally modified version of the BOULE protein (PMID: 27632217).