BPHL Polyclonal antibody

BPHL Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17974-1-AP

Synonyms

VACVASE, Poly(ADP-ribose) glycohydrolase, PARG, MCNAA, EC:3.2.1.143

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Validation Data Gallery

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  WB analysis of human kidney using 17974-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 17974-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17974-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 17974-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 17974-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17974-1-AP (BPHL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human lung using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human heart using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17974-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17974-1-AP (BPHL antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: human kidney tissue, human liver tissue, MCF-7 cells, mouse kidney tissue
• Positive IHC detected in: human kidney tissue, human heart tissue, human lung tissue, human ovary tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17974-1-AP targets BPHL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BPHL fusion protein Ag12085
• Full Name: biphenyl hydrolase-like (serine hydrolase)
• Calculated Molecular Weight: 22 kDa, 32 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC037778
• Gene Symbol: BPHL
• Gene ID (NCBI): 670
• RRID: AB_2274994
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.