BRCA2 Polyclonal antibody

• 

  WB analysis using 29450-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29450-1-AP (BRCA2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 29450-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 29450-1-AP (BRCA2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

BRCA2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29450-1-AP

Publications(8)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

BRCC2, breast cancer 2, early onset, BROVCA2, FACD, FAD

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below

Product Information

29450-1-AP targets BRCA2 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag31064

  Product name: Recombinant human BRCA2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 210-388 aa of NM_000059

  Sequence: IVRNEEASETVFPHDTTANVKSYFSNHDESLKKNDRFIASVTDSENTNQREAASHGFGKTSGNSFKVNSCKDHIGKSMPNVLEDEVYETVVDTSEEDSFSLCFSKCRTKNLQKVRTSKTRKKIFHEANADECEKSKNQVKEKYSFVSEVEPNDTDPLDSNVANQKPFESGSDKISKEVV

• Full Name: breast cancer 2, early onset
• Calculated Molecular Weight: 384 kDa
• Observed Molecular Weight: 384 kDa
• GenBank Accession Number: NM_000059
• Gene Symbol: BRCA2
• Gene ID (NCBI): 675
• RRID: AB_2918309
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P51587
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRCA2, also named as FACD and FANCD1, is involved in double-strand break repair and/or homologous recombination. BRCA2 may participate in S phase checkpoint activation. Defects in BRCA2 are a cause of genetic susceptibility to breast cancer (BC) which also called susceptibility to familial breast-ovarian cancer type 2 (BROVCA2).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Int J Mol Sci; CSB Regulates Pathway Choice in Response to DNA Replication Stress Induced by Camptothecin; Authors - Nicole L Batenburg
human	WB	Aging (Albany NY); A prognostic risk model for programmed cell death and revealing TRIB3 as a promising apoptosis suppressor in renal cell carcinoma; Authors - Qingfeng Fu
human	WB	Nucleic Acids Res; CSB and SMARCAL1 compete for RPA32 at stalled forks and differentially control the fate of stalled forks in BRCA2-deficient cells; Authors - Nicole L Batenburg
human	WB	J Enzyme Inhib Med Chem; Design, synthesis, and evaluation of novel stilbene derivatives that degrade acidic nucleoplasmic DNA-binding protein 1 (And1) and synergize with PARP1 inhibitor in NSCLC cells; Authors - Leyuan Chen
human	WB	Oncol Lett; Olaparib promotes FABP4 expression and reduces antitumor effect in ovarian cancer cells with a BRCA1 mutation; Authors - Wei Huang
human	WB	Am J Physiol Cell Physiol; Synergistic Effects of Olaparib and Palbociclib in Resistant epithelial ovarian cancer; Authors - Shuo Wang