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  • KD/KO Validated

BRD2 Polyclonal antibody

BRD2 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 22236-1-AP

Synonyms

BRD2, bromodomain containing 2, D6S113E, FSH, FSRG1, KIAA9001, NAT, O27.1.1, RING3, RNF3



Tested Applications

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22236-1-AP targets BRD2 in WB, IP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen BRD2 fusion protein Ag17589
Full Name bromodomain containing 2
Calculated Molecular Weight 836 aa, 92 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC063840
Gene Symbol BRD2
Gene ID (NCBI) 6046
RRIDAB_2722524
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for BRD2 antibody 22236-1-APDownload protocol
IP protocol for BRD2 antibody 22236-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nat Med

Prostate cancer-associated SPOP mutations confer resistance to BET inhibitors through stabilization of BRD4.

Authors - Xiangpeng Dai
humanWB

Nat Commun

The H2A.Z and NuRD associated protein HMG20A controls early head and heart developmental transcription programs

Authors - Andreas Herchenröther
human,mouse

Mol Cell

A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis

Authors - Kaiwen Bao
humanWB

Nat Commun

Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer.

Authors - Wenyu Wang
humanWB

Front Oncol

The MYC Paralog-PARP1 Axis as a Potential Therapeutic Target in MYC Paralog-Activated Small Cell Lung Cancer.

Authors - Xing Bian
  • KD Validated
mouseWB

J Neuroinflammation

The BET PROTAC inhibitor dBET6 protects against retinal degeneration and inhibits the cGAS-STING in response to light damage

Authors - Xingfei Zhu