BRD3 Monoclonal antibody, PBS Only (Capture)

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  WB analysis using 60971-1-Ig (same clone as 60971-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60971-1-Ig (BRD3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60971-1-PBS in a different storage buffer formulation.

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  Cytometric bead array sample test of MP51395-1

  Sample test of MP51395-1, BRD3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60971-1-PBS. Detection antibody: 60971-2-PBS.

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  Cytometric bead array standard curve of MP51395-1

  Cytometric bead array standard curve of MP51395-1, BRD3 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 60971-1-PBS. Detection antibody: 60971-2-PBS. Standard:Ag33684. Range: 0.781-100 ng/mL.

BRD3 Monoclonal Antibody for WB, Cytometric bead array, Indirect ELISA

Cat No. 60971-1-PBS

Clone No.1C8C11

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Synonyms

Bromodomain-containing protein 3, KIAA0043, ORFX, RING3 like protein, RING3L

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Product Information

60971-1-PBS targets BRD3 as part of a matched antibody pair:

MP51395-1: 60971-1-PBS capture and 60971-2-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag33684

  Product name: Recombinant human BRD3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-400 aa of BC032124

  Sequence: MSTATTVAPAGIPATPGPVNPPPPEVSNPSKPGRKTNQLQYMQNVVVKTLWKHQFAWPFYQPVDAIKLNLPDYHKIIKNPMDMGTIKKRLENNYYWSASECMQDFNTMFTNCYIYNKPTDDIVLMAQALEKIFLQKVAQMPQEEVELLPPAPKGKGRKPAAGAQSAGTQQVAAVSSVSPATPFQSVPPTVSQTPVIAATPVPTITANVTSVPVPPAAAPPPPATPIVPVVPPTPPVVKKKGVKRKADTTTPTTSAITASRSESPPPLSDPKQAKVVARRESGGRPIKPPKKDLEDGEVPQHAGKKGKLSEHLRYCDSILREMLSKKHAAYAWPFYKPVDAEALELHDYHDIIKHPMDLSTVKRKMDGREYPDAQGFAADVRLMFSNCYKYNPPDHEVVAM

• Full Name: bromodomain containing 3
• Calculated Molecular Weight: 556 aa, 61 kDa
• Observed Molecular Weight: 95-105 kDa
• GenBank Accession Number: BC032124
• Gene Symbol: BRD3
• Gene ID (NCBI): 8019
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q15059
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BRD3 was belongs to the BET family that a small group of unique, highly conserved bromodomain-containing genes with four members in mouse and human. There are BRD2, BRD3, BRD4 and BRDT. And BET family proteins all localize in the nucleus, and contain two tandems N-terminal BRDs, an extra-terminal (ET) domain and a more divergent C-terminal recruitment domain (PMID:25849938). BRD2, BRD3, and BRD4 play key roles in transcription of genes required for both inflammation and cancer. Northern hybridization revealed that BRD3 is most abundant in testis, ovary, placenta, uterus, and brain(PMID:15261828; PMID:27451123).