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BRD4 Polyclonal antibody, PBS Only (28486-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 28486-1-AP (same clone as 28486-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
WB analysis using 28486-1-AP (same clone as 28486-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
WB analysis of Jurkat using 28486-1-AP (same clone as 28486-1-PBS)
A549 cells were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
WB analysis using 28486-1-AP (same clone as 28486-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
WB analysis using 28486-1-AP (same clone as 28486-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 28486-1-AP (BRD4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
IP experiment of A549 using 28486-1-AP (same clone as 28486-1-PBS)
IP result of anti-BRD4 (IP:28486-1-AP, 4ug; Detection:28486-1-AP 1:1000) with A549 cells lysate 800 ug. This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 28486-1-AP (same clone as 28486-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28486-1-AP (BRD4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 28486-1-AP (same clone as 28486-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using BRD4 antibody (28486-1-AP) at dilution of 1:600 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 28486-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

BRD4 Polyclonal antibody, PBS Only

Name: BRD4 Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 28486-1-PBS

BRD4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 28486-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

BRD 4, bromodomain containing 4, Bromodomain-containing protein 4, HUNKI, MCAP

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

28486-1-PBS targets BRD4 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	bromodomain containing 4
Calculated Molecular Weight	1362 aa, 152 kDa
Observed Molecular Weight	200 kDa
GenBank Accession Number	BC035266
Gene Symbol	BRD4
Gene ID (NCBI)	23476
RRID	AB_2918170
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60885
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

BRD4 is a member of the BET (bromodomain and extra terminal domain) family, which also includes BRD2, BRD3, and BRDT. BRD4, similar to other BET family members, contains two bromodomains that recognize acetylated lysine residues. BRD4 also has an extended C-terminal domain with little sequence homology to other BET family members. BRD4 has some isoforms, the long isoform BRD4-L has an extended C terminus, while the short isoform BRD4-S lacks this C-terminal extension. This antibody detects the small and the long isoform of BRD4, the molecular weight range of BRD4-S and BRD4-L is 110 kDa to 200 kDa (PMID: 32203489).