CoraLite®594-conjugated BRD8 Monoclonal antibody

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• KD/KO Validated

BRD8 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

5B6D6

Cat no : CL594-60121

Synonyms

BRD8, bromodomain containing 8, p120, SMAP, SMAP2, TrCP120

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-60121

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-60121 (BRD8 antibody) at dilution of 1:100.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-60121 targets BRD8 in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BRD8 fusion protein Ag0790
• Full Name: bromodomain containing 8
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC008039
• Gene Symbol: BRD8
• Gene ID (NCBI): 10902
• RRID: AB_2883425
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

BRD8 is one of the bromodomain-containing proteins, which is an acetylated lysine- binding domain and thought to be involved in regulation of protein acetylation and/or HAT (histone acetyl transferase) activity [PMID: 12963728]. It is also reported to be an accessory subunit of NuA4 HAT complex (also known as TRRAP/TIP60 complex) through biochemical purification [PMID: 16049979]. In addition, it can bind to thyroid hormone receptor-ß and/or retinoid X receptor, and can act as a coactivator of the nuclear hormone receptor-mediated transcription in reporter assays [PMID: 10517671].