BRE Monoclonal antibody

BRE Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

2E5A5

Cat no : 66356-1-Ig

Synonyms

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2

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Validation Data Gallery

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  WB analysis of fetal human brain using 66356-1-Ig

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 66356-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 66356-1-Ig (BRE Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 66356-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 66356-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66356-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 66356-1-Ig

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 66356-1-Ig (BRE Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 66356-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRE antibody (66356-1-Ig, Clone: 2E5A5 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive WB detected in: fetal human brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue, human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

66356-1-Ig targets BRE in WB, IHC, IF, ELISA applications and shows reactivity with human, rat, mouse samples.

• Tested Reactivity: human, rat, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: BRE fusion protein Ag2256
• Full Name: brain and reproductive organ-expressed (TNFRSF1A modulator)
• Calculated Molecular Weight: 383 aa, 44 kDa
• Observed Molecular Weight: 42-47 kDa
• GenBank Accession Number: BC001251
• Gene Symbol: BRE
• Gene ID (NCBI): 9577
• RRID: AB_2881735
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.