BRE Monoclonal antibody

BRE Monoclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

CloneNo.

2E5A5

Cat no : 66356-1-Ig

Synonyms

BABAM2, BRCA1 A complex subunit BRE, BRCC4, BRCC45, BRE, BRISC and BRCA1-A complex member 2



Tested Applications

Positive WB detected infetal human brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue, human gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IF experiment? We recommend our CoraLite®488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66356-1-Ig targets BRE in WB, IHC, IF,ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen BRE fusion protein Ag2256
Full Name brain and reproductive organ-expressed (TNFRSF1A modulator)
Calculated molecular weight 383 aa, 44 kDa
Observed molecular weight 42-47 kDa
GenBank accession numberBC001251
Gene symbol BRE
Gene ID (NCBI) 9577
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

BRE, also named as BRCC45, is a death receptor-associated antiapoptotic protein, which is also involved in DNA-damage repair, and K63-specific deubiquitination. BRE and its XIAP-sustaining mechanism could represent novel targets for anti-cancer therapy. BRE may regulate TNF-alpha signaling through its interactions with TNFRSF1A. It plays a role in homeostasis or cellular differentiation in cells of neural, epithelial and germline origins. BRE gene has four isoforms with MW 42-47 kDa.

Protocols

Product Specific Protocols
WB protocol for BRE antibody 66356-1-IgDownload protocol
IHC protocol for BRE antibody 66356-1-IgDownload protocol
IF protocol for BRE antibody 66356-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols