Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24436-1-AP targets BRIP1(N-terminal) in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19907 Product name: Recombinant human BRIP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-342 aa of BC101472 Sequence: MSSMWSEYTIGGVKIYFPYKAYPSQLAMMNSILRGLNSKQHCLLESPTGSGKSLALLCSALAWQQSLSGKPADEGVSEKAEVQLSCCCACHSKDFTNNDMNQGTSRHFNYPSTPPSERNGTSSTCQDSPEKTTLAAKLSAKKQASIYRDENDDFQVEKKRIRPLETTQQIRKRHCFGTEVHNLDAKVDSGKTVKLNSPLEKINSFSPQKPPGHCSRCCCSTKQGNSQESSNTIKKDHTGKSKIPKIYFGTRTHKQIAQITRELRRTAYSGVPMTILSSRDHTCVHPEVVGNFNRNEKCMELLDGKNGKSCYFYHGVHKISDQHTLQTFQGMCKAWDIEELVS Predict reactive species |
| Full Name | BRCA1 interacting protein C-terminal helicase 1 |
| Calculated Molecular Weight | 1249 aa, 141 kDa |
| Observed Molecular Weight | 140-150 kDa |
| GenBank Accession Number | BC101472 |
| Gene Symbol | BACH1/BRIP1 |
| Gene ID (NCBI) | 83990 |
| RRID | AB_2879548 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BX63 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BRIP1 also named as BACH1, FANCJ or ATP dependent RNA helicase BRIP1 is a 1249 amino acid protein, which contains one helicase ATP-binding domain and belongs to the DEAD box heilicase family, DEAH subfamily. BRIP1 contains nuclear localization signal and is ubiquitously expressed with highest levels in testis, an expression pattern similar to that of BRCA1. BRIP1 is required to resolve the secondary structures of guanine-rich DNA that occasionally form during lagging-strand DNA synthesis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BRIP1(N-terminal) antibody 24436-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PARP1 (14D1) Rabbit Monoclonal Antibody, catalog number 24436-1-AP, has 4 total citations. These appear in Molecular Cell, Pharmacological Reports, Cancer Letters, and Cell Reports. The associated research fields include DNA repair and homologous recombination, Nrf2-mediated neuroprotection in cerebral ischemia, neuroblastoma tumor initiation, and DNA replication/replisome dynamics.
| Species | Application | Title |
|---|---|---|
Mol Cell A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination. | ||
Pharmacol Rep Integrated computational and experimental approach to identify Nrf2-regulated molecular targets in cerebral ischemia. | ||
Cancer Lett A human stem cell model of neuroblastoma identifies four chromosome 17q genes that drive tumor initiation and progression. | ||
Cell Rep FANCJ and RTEL1 facilitate pre-replication complex disassembly following replisome collision. |
Reviews
What customers say
The single review rates the antibody 4 out of 5 stars for Western Blot on MCF7 cells at a 1:1000 dilution. The reviewer noted it shows more background than some other Proteintech antibodies and appears to detect two bands, but confirmed the correct band is clearly visible at around 140 kDa. Overall, the antibody works but with somewhat higher background than expected.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Adrianna (Verified Customer) (10-31-2022) | This antibody is not as good as some of the other Proteintech antibodies (it has more background) and it looks like I am detecting two bands, however I can clearly see the correct band at around 140 kDa.
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