MCPH/BRIT1 Polyclonal antibody

• Featured Product
• KD/KO Validated

MCPH/BRIT1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (1)

Cat no : 11962-1-AP

Synonyms

BRIT1, MCPH1, MCT, Microcephalin, microcephalin 1

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Validation Data Gallery

• 

  WB analysis of HeLa using 11962-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MDA-MB-453s using 11962-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 11962-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 11962-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11962-1-AP (BRIT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 11962-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11962-1-AP (BRIT1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 11962-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney slide using 11962-1-AP (BRIT1 Antibody) at dilution of 1:50.

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Tested Applications

• Positive WB detected in: HeLa cells, L02 cells, MDA-MB-453s cells, mouse ovary tissue
• Positive IHC detected in: human prostate cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 1 publications below

Product Information

11962-1-AP targets MCPH/BRIT1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MCPH/BRIT1 fusion protein Ag2567
• Full Name: microcephalin 1
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 66 kDa, 100 kDa
• GenBank Accession Number: BC030702
• Gene Symbol: MCPH1
• Gene ID (NCBI): 79648
• RRID: AB_2142989
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Primary microcdphaly refers to the clinical finding of a head circumference less than 3 standard deviations(SD) below the age- and sex-related mean, present at birth. MCPH1, the fisrt gene identified as causative for primary microcephaly, encodes a multifunctional protein that notably is linked to DNA damage checkpoint, DNA repair by homologous recombination and DNA transcription. MCPH1 is associated with premature chromosome condensation in particular. There are three mainly isoforms encoded by MCPH1 gene, full-length MCPH1 (MCPH1-FL) (~100kd), lacking the C-terminal BRCTisoform 2(~70kd) and lacking middle region isoform 3(~50kd).(PMID: 22952573 )

Publications

Species	Application	Title
human	WB	FASEB J; Phosphorylation of MCPH1 isoforms during mitosis followed by isoform-specific degradation by APC/C-CDH1.; Authors - Stephanie K Meyer ; KD Validated
human	WB, IHC	Stem Cell Res; Generation of a MCPH1 knockout human embryonic stem cell line by CRISPR/Cas9 technology.; Authors - Zerui Wang ; KO Validated
human	WB	FEBS J; Mitotic entry upon Topo II catalytic inhibition is controlled by Chk1 and Plk1.; Authors - Maria Arroyo ; KD Validated
human	WB	Genes (Basel); MCPH1 Lack of Function Enhances Mitotic Cell Sensitivity Caused by Catalytic Inhibitors of Topo II.; Authors - María Arroyo ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Paul (Verified Customer) (11-10-2022)

I compared several commercial antibodies for MCPH1 and this was the only one that was able to detect both isoforms in Western blotting. Importantly, these bands disappeared after treatment of cells with MCPH1 siRNA.

• Applications: Western Blot
• Cell Tissue Type: Human cell lines