BRN3A Polyclonal antibody, PBS Only

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• BRN3A Polyclonal antibody, PBS Only (32257-1-PBS)

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  WB analysis using 32257-1-AP (same clone as 32257-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 32257-1-AP (BRN3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 32257-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 32257-1-AP (same clone as 32257-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 32257-1-AP (BRN3A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32257-1-PBS in a different storage buffer formulation.

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  IF Staining of rat eye using 32257-1-AP (same clone as 32257-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat eye tissue using BRN3A antibody (32257-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 32257-1-PBS in a different storage buffer formulation.

BRN3A Polyclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 32257-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Brain-3A, Brain-specific homeobox/POU domain protein 3A, Brn-3A, Homeobox/POU domain protein RDC-1, Oct-T1

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Product Information

32257-1-PBS targets BRN3A in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag35800

  Product name: Recombinant human BRN3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 87-265 aa of NM_006237

  Sequence: SVPCTSTSTVPLAHHHHHHHHHQALEPGDLLDHISSPSLALMAGAGGAGAAAGGGGAHDGPGGGGGPGGGGGPGGGPGGGGGGGPGGGGGGPGGGLLGGSAHPHPHMHSLGHLSHPAAAAAMNMPSGLPHPGLVAAAAHHGAAAAAAAAAAGQVAAASAAAAVVGAAGLASICDSDTDP

• Full Name: POU class 4 homeobox 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: NM_006237
• Gene Symbol: POU4F1
• Gene ID (NCBI): 5457
• RRID: AB_3742554
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q01851
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BRN3A (Brain-specific Homeobox/POU domain protein 3A, also known as POU4F1) is a member of the class IV POU-domain transcription factor family. It plays a core regulatory role primarily in the development and survival of the nervous system and sensory neurons. By recognizing specific DNA sequences, it activates downstream target genes and is essential for the generation, differentiation, and maintenance of sensory ganglion neurons, including those of the trigeminal, vestibular, and auditory systems. Additionally, it is also involved in regulating cell proliferation and apoptosis in tumors such as breast cancer. It has 2 isoforms with the molecular mass of 33 kDa and 43 kDa.