BRP16 Polyclonal antibody, PBS Only

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• BRP16 Polyclonal antibody, PBS Only (20169-1-PBS)

BRP16 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 20169-1-PBS Copied!

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

C8orf30A, Brain protein 16, HGH1

• 

  WB analysis using 20169-1-AP (same clone as 20169-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20169-1-AP (BRP16 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20169-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 20169-1-AP (same clone as 20169-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using BRP16 antibody (20169-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 20169-1-PBS in a different storage buffer formulation.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

20169-1-PBS targets BRP16 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13998

  Product name: Recombinant human BRP16 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 41-390 aa of BC003035

  Sequence: AVRHVLALTGCGPGRALLAGQAALLQALMELAPASAPARDAARALVNLAADPGLHETLLAADPGLPARLMGRALDPQWPWAEEAAAALANLSREPAPCAALMAALAAAEPADSGLERLVRALCTPGYNARAPLHYLAPLLSNLSQRPAARAFLLDPDRCVVQRLLPLTQYPDSSVRRGGVVGTLRNCCFEHRHHEWLLGPEVDILPFLLLPLAGPEDFSEEEMERLPVDLQYLPPDKQREPDADIRKMLVEAIMLLTATAPGRQQVRDQGAYLILRELHSWEPEPDVRTACEKLIQVLIGDEPERGMENLLEVQVPEDVEQQLQQLDCREQEQLERELAPEPWVERATPT

• Full Name: chromosome 8 open reading frame 30A
• Calculated Molecular Weight: 390 aa, 42 kDa
• Observed Molecular Weight: 42-50 kDa
• GenBank Accession Number: BC003035
• Gene Symbol: BRP16
• Gene ID (NCBI): 51236
• RRID: AB_2878652
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BTY7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

HGH1 (HGH1 homolog; also known as BRP16/brain protein 16, FAM203A or C8orf30A) is a 390-residue, armadillo-repeat protein of the HGH1 family that acts as a co-chaperone of eukaryotic elongation factor 2 (eEF2) (PMID: 30876804). In yeast, it binds the dynamic central domain III of nascent eEF2 through a bipartite interface and recruits the chaperonin TRiC/CCT to the C-terminal module, and without HGH1 a substantial fraction of newly synthesized eEF2 is degraded or aggregates. HGH1 also cooperates with the Hsp90 co-chaperone Cns1 in a quaternary complex with eEF2 and Hsp90 (PMID: 30876805), and hgh1-deleted yeast contain eEF2 that lacks the diphthamide modification (PMID: 39825589). In human breast cancer cells, HGH1 depletion reduces global translation efficiency and proliferation (PMID: 38844963). HGH1 is upregulated in breast tumors, predicts poor prognosis and promotes proliferation, migration and invasion through PI3K/AKT/NF-kappa B signaling (PMID: 40256249).