BRPF1 Polyclonal antibody

BRPF1 Polyclonal Antibody for WB, FC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

Cat no : 30186-1-AP

Synonyms

BR140, BRPF1, Peregrin, Protein Br140

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Validation Data Gallery

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  WB analysis using 30186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30186-1-AP (BRPF1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 30186-1-AP

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human BRPF1 (30186-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

• Positive WB detected in: HeLa cells, PC-12 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30186-1-AP targets BRPF1 in WB, FC, ELISA applications and shows reactivity with Human, rat samples.

• Tested Reactivity: Human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BRPF1 fusion protein Ag32828
• Full Name: bromodomain and PHD finger containing, 1
• Calculated Molecular Weight: 1220 aa, 138 kDa
• Observed Molecular Weight: 140 kDa
• GenBank Accession Number: BC053851
• Gene Symbol: BRPF1
• Gene ID (NCBI): 7862
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BRPF1 (bromodomain and PHD finger-containing protein 1) is also named as BR140. BRPF1 is scaffold subunit of various histone acetyltransferase (HAT) complexes, such as the MOZ/MORF and HBO1 complexes, which have a histone H3 acetyltransferase activity (PMID:16387653, PMID:24065767, PMID:27939640). BRPF1 plays a key role in HBO1 complex by directing KAT7/HBO1 specificity towards histone H3 'Lys-14' acetylation (H3K14ac) (PMID:24065767). And BRPF1 positively regulates the transcription of RUNX1 and RUNX2 (PMID:18794358).