BSAP,PAX5 Monoclonal antibody

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  WB analysis of Ramos using 60349-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Daudi using 60349-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 60349-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human tonsillitis using 60349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Ramos using 60349-1-Ig

  1X10^6 Ramos cells were intracellularly stained with 0.4 ug Anti-Human BSAP,PAX5 (60349-1-Ig, Clone:2H2D6) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

BSAP,PAX5 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 60349-1-Ig

Clone No.2H2D6

Publications(1)

Reviews (1)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF, FC (Intra), IP, ELISA

Synonyms

PAX5, 2H2D6, B-cell-specific transcription factor, paired box 5, Paired box protein Pax 5

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Tested Applications

• Positive WB detected in: Ramos cells, Daudi cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Ramos cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

60349-1-Ig targets BSAP,PAX5 in WB, IHC, IF, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BSAP,PAX5 fusion protein Ag16023
• Full Name: paired box 5
• Calculated Molecular Weight: 391 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC156927
• Gene Symbol: PAX5
• Gene ID (NCBI): 5079
• RRID: AB_2881458
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q02548
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.

Publications

Species	Application	Title
human	WB,IP,IHC,IF	Cell Death Dis; The MBNL1/circNTRK2/PAX5 pathway regulates aerobic glycolysis in glioblastoma cells by encoding a novel protein NTRK2-243aa; Authors - Yubo Zhao ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kenzo (Verified Customer) (03-28-2024)

This antibody reacted mouse lymphocytes in tonsil and worked well for immunolabeling.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: Tonsil