BSAP,PAX5 Monoclonal antibody

BSAP,PAX5 Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

2H2D6

Cat no : 60349-1-Ig

Synonyms

BSAP, BSAP,PAX5, paired box 5, Paired box protein Pax 5, PAX5

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Validation Data Gallery

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  WB analysis of Ramos using 60349-1-Ig

  Ramos cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Daudi using 60349-1-Ig

  Daudi cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Raji using 60349-1-Ig

  Raji cells were subjected to SDS PAGE followed by western blot with 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 60349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60349-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60349-1-Ig (BSAP,PAX5 Antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: Ramos cells, Daudi cells, Raji cells
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

Sample-dependent, check data in validation data gallery

Product Information

60349-1-Ig targets BSAP,PAX5 in WB, IHC,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: BSAP,PAX5 fusion protein Ag16023
• Full Name: paired box 5
• Calculated molecular weight: 391 aa, 42 kDa
• Observed molecular weight: 42 kDa
• GenBank accession number: BC156927
• Gene symbol: PAX5
• Gene ID (NCBI): 5079
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PAX5, also named B-cell specific activator protein (BSAP), is a member of the highly conserved paired box (PAX)-domain family of transcription factors. PAX5 is essential in normal B-cell lymphopoiesis. It is expressed in pro-B, pre-B and mature B lymphocytes but not in plasma cells. PAX5 is a common target in leukemogenesis of B-ALL ( B-lineage acute lymphoblastic leukemia) along with CDKN2A. Owing to its frequent deletion in B-ALL, PAX5 could be used as one of the molecular markers in diagnosis and monitoring of the disease. This antibody is specific to PAX5. It will not cross react with other PAX members.