BTF3 Polyclonal antibody

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  WB analysis using 15415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15415-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15415-1-AP (BTF3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15415-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15415-1-AP (BTF3 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 15415-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using BTF3 antibody (15415-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

BTF3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15415-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

OK/SW-cl.8, Nascent polypeptide-associated complex subunit beta, NAC-beta, NACB, BTF3b

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Tested Applications

• Positive WB detected in: A549 cells, HT-29 cells, HeLa cells, HuH-7 cells, RAW 264.7 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15415-1-AP targets BTF3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BTF3 fusion protein Ag7701
• Full Name: basic transcription factor 3
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 18 kDa, 23 kDa
• GenBank Accession Number: BC008062
• Gene Symbol: BTF3
• Gene ID (NCBI): 689
• RRID: AB_3669206
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P20290
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Basic transcription factor 3 (BTF3), also known as nascent-polypeptide-associated complex (NAC) beta (NACB), belongs to the NAC-beta family. BTF3 serves as an oncogene and convey worse prognosis in gastric cancer, pancreatic cancer, osteosarcoma, cervical cancer, hypopharyngeal squamous cell carcinoma, prostate cancer , breast cancer. Due to alternative splicing, BTF3 is present in two different isoforms, BTF3a and BTF3b. BTF3a is the transcriptionally active form of BTF3, while the isoform lacking the first 44 amino acids of the BTF3a N-terminus, BTF3b, is transcriptionally inactive, two isoforms of BTF3, BTF3a and BTF3b corresponding to 23kDa and 18kDa(PMID: 23029305).