Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody, PBS Only

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  FC experiment of mouse splenocytes using 98246-2-RR (same clone as 98246-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed. This data was developed using the same antibody clone with 98246-2-PBS in a different storage buffer formulation.

• 

  FC experiment of mouse splenocytes using 98246-2-RR (same clone as 98246-2-PBS)

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98246-2-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98246-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98246-2-RR against Mouse BTLA/CD272 were performed. The affinity constant is 0.441 nM.

BTLA/CD272 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98246-2-PBS

Clone No.242133A1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD272, Btla, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133A1

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Product Information

98246-2-PBS targets BTLA/CD272 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: B and T lymphocyte associated
• Calculated Molecular Weight: 34kDa
• GenBank Accession Number: AAI08964.1
• Gene Symbol: Btla
• Gene ID (NCBI): 208154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q32MV9
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

BTLA, also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.