Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody

• 

  FC experiment of mouse splenocytes using 98246-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD45R (B220) (RA3-6B2) (APC-65139, Clone: RA3-6B2). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98246-2-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse BTLA/CD272 Rabbit Recombinant Antibody (98246-2-RR, Clone: 242133A1)(red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98246-2-RR

  Biolayer interferometry (BLl) kinetic assays of 98246-2-RR against Mouse BTLA/CD272 were performed. The affinity constant is 0.441 nM.

BTLA/CD272 Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98246-2-RR

Clone No.242133A1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

FC

Synonyms

CD272, Btla, B- and T-lymphocyte-associated protein, B- and T-lymphocyte attenuator, 242133A1

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Tested Applications

• Positive FC detected in: mouse splenocytes

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

98246-2-RR targets BTLA/CD272 in FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: B and T lymphocyte associated
• Calculated Molecular Weight: 34kDa
• GenBank Accession Number: AAI08964.1
• Gene Symbol: Btla
• Gene ID (NCBI): 208154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q32MV9
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

BTLA, also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.