FcZero-rAb® Biotin Anti-Human BTLA/CD272 Rabbit Recombinant Antibody

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• FcZero-rAb® Biotin Anti-Human BTLA/CD272 Rabbit Recombinant Antibody (Biotin-FcA98096)

• Zero Fc receptor binding
• Site-specific conjugation
• One universal isotype

BTLA/CD272 FcZero-rAb^® Recombinant Antibody for FC, ELISA

Cat No. Biotin-FcA98096 Copied!

Clone No. 241318H5

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC, ELISA

Synonyms

BTLA, B and T lymphocyte attenuator, B and T lymphocyte associated, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein

• 

  FC experiment of human PBMCs using Biotin-FcA98096

  1x10^6 human PBMCs were surface stained with FcZero-rAb™ APC Anti-Human CD3, and 0.25 ug Biotin Anti-Human BTLA/CD272 Rabbit RecAb (Biotin-FcA98096, Clone: 241318H5) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of human PBMCs using Biotin-FcA98096

  1x10^6 human PBMCs were surface stained with 0.25 ug Biotin Anti-Human BTLA/CD272 Rabbit RecAb (Biotin-FcA98096, Clone: 241318H5) (red) or FcZero-rAb™ Biotin Rabbit IgG Isotype Control Recombinant Antibody (Biotin-FcA98136, Clone: 240953C9) (blue), and Streptavidin-PE Conjugate (PE-PF00030). Cells were not fixed.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-FcA98096 targets BTLA/CD272 in FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant Protein

• Full Name: B and T lymphocyte associated
• Calculated Molecular Weight: 289 aa, 33 kDa
• GenBank Accession Number: BC107091
• Gene Symbol: BTLA
• Gene ID (NCBI): 151888
• RRID: AB_3746913
• Conjugate: Biotin
• Excitation/Emission Maxima Wavelengths: -
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q7Z6A9
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

BTLA ( B and T lymphocyte attenuator), also known as CD272, is a member of the CD28 superfamily and is a type I membrane glycoprotein identified as an inhibitory receptor (PMID: 33859648). BTLA is extensively expressed in lymph nodes, thymus, and spleen, with little or no expression in organs such as the heart, kidney, brain, and liver (PMID: 33859648). Among immune cells, BTLA is primarily expressed in B and T cells, with higher expression in B cells compared to T cells in the mouse spleen (PMID: 33859648). BTLA plays a crucial role in regulating stimulatory and inhibitory signals in immune responses.