Tested Applications
| Positive WB detected in | mouse testis tissue, K-562 cells, HeLa cells, human testis tissue |
| Positive IHC detected in | human cervical cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SKOV-3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11504-2-AP targets BUB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2053 Product name: Recombinant human BUB1B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 719-1050 aa of BC018739 Sequence: QSPWCSQYRRQLLKSLPELSASAELCIEDRPMPKLEIEKEIELGNEDYCIKREYLICEDYKLFWVAPRNSAELTVIKVSSQPVPWDFYINLKLKERLNEDFDHFCSCYQYQDGCIVWHQYINCFTLQDLLQHSEYITHEITVLIIYNLLTIVEMLHKAEIVHGDLSPRCLILRNRIHDPYDCNKNNQALKIVDFSYSVDLRVQLDVFTLSGFRTVQILEGQKILANCSSPYQVDLFGIADLAHLLLFKEHLQVFWDGSFWKLSQNISELKDGELWNKFFVRILNANDEATVSVLGELAAEMNGVFDTTFQSHLNKALWKVGKLTSPGALLFQ Predict reactive species |
| Full Name | budding uninhibited by benzimidazoles 1 homolog beta (yeast) |
| Calculated Molecular Weight | 1050 aa, 120 kDa |
| Observed Molecular Weight | 120-130 kDa |
| GenBank Accession Number | BC018739 |
| Gene Symbol | BubR1 |
| Gene ID (NCBI) | 701 |
| RRID | AB_2066073 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60566 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3, and SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID: 22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications, including acetylation(PMID: 22167194). Defects in BUB1B are the cause of the premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1). Research has shown that a BUB1B isoform with a measured MW of about 65 kDa (PMID: 34620840).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for BUB1B antibody 11504-2-AP | Download protocol |
| IHC protocol for BUB1B antibody 11504-2-AP | Download protocol |
| WB protocol for BUB1B antibody 11504-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BUB1B Polyclonal antibody (Cat# 11504-2-AP) has 30 citations across journals including Nature Communications, Oncogene, Nucleic Acids Research, Advanced Science, Cancer Letters, FASEB Journal, Journal of Biological Chemistry, Scientific Reports, Phytomedicine, and Frontiers in Immunology. Research fields span spindle assembly checkpoint, cell cycle regulation, oocyte meiosis, and diverse cancers (breast, lung, colorectal, glioblastoma, hepatocellular, pancreatic, endometrial, cervical). Cited applications include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun CENP-F-dependent DRP1 function regulates APC/C activity during oocyte meiosis I | ||
J Adv Res Tankyrase activity is essential for asymmetric division and chromosome segregation in oocyte meiosis | ||
Adv Sci (Weinh) MIS12 Is Required for Kinetochore-Microtubule Attachment in Oocyte Meiosis | ||
Cancer Lett Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells. | ||
Int J Biol Sci The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer. |
Reviews
What customers say
Both reviewers gave this antibody a perfect 5-star rating. One user reported excellent performance across Western Blot, Immunohistochemistry, and Immunofluorescence at a 1:1000 dilution, describing it as a "very good antibody" that "works good." The other reviewer also used it for Western Blot at the same dilution and simply called it a "good antibody." Overall, users are satisfied with its reliability across multiple applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mounika (Verified Customer) (09-08-2026) | very good antibody, works good
|
Sai Sindhura (Verified Customer) (08-19-2026) | Good antibody
|
