BUB1B Polyclonal antibody

• Featured Product
• KD/KO Validated

BUB1B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 11504-2-AP

Synonyms

BubR1, MAD3/BUB1-related protein kinase, hBUBR1, EC:2.7.11.1, BUB1beta

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Validation Data Gallery

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  WB analysis of HeLa using 11504-2-AP

  WB result of BubR1 antibody (11504-2-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-BubR1 transfected HeLa cells.

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  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 11504-2-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11504-2-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11504-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 11504-2-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 11504-2-AP (BubR1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cervical cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 11504-2-AP (BubR1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human colon cancer using 11504-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11504-2-AP (BubR1 antibody at dilution of 1:200 (under 40x lens).

• 

  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using BubR1 antibody (11504-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of SKOV-3 using 11504-2-AP

  Immunofluorescent analysis of SKOV-3 cells using 11504-2-AP (BubR1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse testis tissue, K-562 cells, HeLa cells, human testis tissue
• Positive IHC detected in: human cervical cancer tissue, human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SKOV-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 11 publications below
• IHC: See 3 publications below
• IF: See 4 publications below

Product Information

11504-2-AP targets BUB1B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BUB1B fusion protein Ag2053
• Full Name: budding uninhibited by benzimidazoles 1 homolog beta (yeast)
• Calculated Molecular Weight: 1050 aa, 120 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC018739
• Gene Symbol: BubR1
• Gene ID (NCBI): 701
• RRID: AB_2066073
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

BUB1B(Mitotic checkpoint serine/threonine-protein kinase BUB1 beta), also named as BUBR1, MAD3Land SSK1, belongs to the BUB1 subfamily. Human BUB1B is a 120 kDa protein that plays a central role in the spindle assembly checkpoint (SAC), the evolutionary conserved and self-regulatory system of higher organisms that monitors and repairs defects in chromosome segregation in mitotic cells(PMID:22201036). In addition to phosphorylation, BUB1B is also subjected to posttranslational modifications including acetylation(PMID:22167194). Defects in BUB1B are the cause of premature chromatid separation trait (PCS) and mosaic variegated aneuploidy syndrome type 1 (MVA1).

Publications

Species	Application	Title
human	WB	Nucleic Acids Res; ULK1 phosphorylates Mad1 to regulate spindle assembly checkpoint.; Authors - Fengjie Yuan
human	WB	Oncogene; KPNB1 inhibition disrupts proteostasis and triggers unfolded protein response-mediated apoptosis in glioblastoma cells.; Authors - Zhi-Chuan Zhu
human	WB	Cancer Lett; Pseudolaric acid B induces mitotic arrest and apoptosis in both 5-fluorouracil-sensitive and -resistant colorectal cancer cells.; Authors - Chuangyu Wen
human	WB	Bioorg Chem; Design, synthesis and antitumor evaluation of novel chiral diaryl substituted azetidin-2-one derivatives as tubulin polymerization inhibitors.; Authors - Yuru Liang
human	WB	Eur J Med Chem; Design, synthesis, biological evaluation and cocrystal structures with tubulin of chiral β-lactam bridged combretastatin A-4 analogues as potent antitumor agents.; Authors - Pengfei Zhou
human	WB	Int J Biol Sci; The Role of CHK1 Varies with the Status of Oestrogen-receptor and Progesterone-receptor in the Targeted Therapy for Breast Cancer.; Authors - Wei Xu