BUD31 Recombinant antibody

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  WB analysis using 85336-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85336-1-RR (BUD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85336-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85336-1-RR (BUD31 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85336-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85336-1-RR (BUD31 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 85336-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85336-1-RR (BUD31 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85336-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85336-1-RR against Human BUD31 were performed. The affinity constant is below 1 pM.

BUD31 Recombinant Antibody for WB, IHC, ELISA

Cat No. 85336-1-RR

Clone No.242551D3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

242551D3, EDG 2, EDG2, fSAP17, G10

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Tested Applications

• Positive WB detected in: Jurkat cells, mouse brain tissue, Raji cells, K-562 cells, U-251 cells, rat heart tissue, fetal human brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85336-1-RR targets BUD31 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: BUD31 fusion protein Ag2373
• Full Name: BUD31 homolog (S. cerevisiae)
• Calculated Molecular Weight: 144 aa, 17 kDa
• Observed Molecular Weight: 17 kDa
• GenBank Accession Number: BC022821
• Gene Symbol: BUD31
• Gene ID (NCBI): 8896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P41223
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.