BXDC2 Polyclonal antibody

BXDC2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17295-1-AP

Synonyms

BXDC 2, BRIX1, Brix domain-containing protein 2, BRIX

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Validation Data Gallery

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  WB analysis of human liver using 17295-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 17295-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17295-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 17295-1-AP

  IP result of anti-BXDC2 (IP:17295-1-AP, 4ug; Detection:17295-1-AP 1:700) with HEK-293 cells lysate 2400 ug.

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  IHC staining of human pancreas using 17295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17295-1-AP (BXDC2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas using 17295-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 17295-1-AP (BXDC2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17295-1-AP

  Immunofluorescent analysis of HepG2 cells, using BXDC2 antibody 17295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: human liver tissue, HEK-293 cells, HeLa cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17295-1-AP targets BXDC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BXDC2 fusion protein Ag11174
• Full Name: brix domain containing 2
• Calculated Molecular Weight: 353 aa, 41 kDa
• Observed Molecular Weight: 41 kDa
• GenBank Accession Number: BC001546
• Gene Symbol: BXDC2
• Gene ID (NCBI): 55299
• RRID: AB_1939420
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.