human liver tissue were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
human liver tissue were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17295-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
HeLa cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HEK-293 using 17295-1-AP
IP result of anti-BXDC2 (IP:17295-1-AP, 4ug; Detection:17295-1-AP 1:700) with HEK-293 cells lysate 2400 ug.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:500-1:3000
Immunoprecipitation (IP)
IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)
IHC : 1:20-1:200
Immunofluorescence (IF)/ICC
IF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
human liver tissue were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 17295-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 17295-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 17295-1-AP (BXDC2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human pancreas using 17295-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 17295-1-AP (BXDC2 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human pancreas using 17295-1-AP
Immunohistochemical analysis of paraffin-embedded human pancreas using 17295-1-AP (BXDC2 antibody) at dilution of 1:100 (under 40x lens).
IP Figures
IP experiment of HEK-293 using 17295-1-AP
IP result of anti-BXDC2 (IP:17295-1-AP, 4ug; Detection:17295-1-AP 1:700) with HEK-293 cells lysate 2400 ug.
IF/ICC Figures
IF Staining of HepG2 using 17295-1-AP
Immunofluorescent analysis of HepG2 cells, using BXDC2 antibody 17295-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
BXDC2 Polyclonal antibody
Catalog Number
17295-1-AP
Citations
1
Dilutions
WB : 1:500-1:3000 IP : 0.5-4.0 ug for IP and 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate for WB IHC : 1:20-1:200 IF/ICC : 1:10-1:100
Applications
WB, IHC, IF/ICC, IP, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
