BZW1 Polyclonal antibody

• Featured Product
• KD/KO Validated

BZW1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 19917-1-AP

Synonyms

Nbla10236, KIAA0005, eIF5-mimic protein 2, BZAP45, Basic leucine zipper and W2 domain-containing protein 1

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Validation Data Gallery

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  WB analysis of MCF-7 using 19917-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 19917-1-AP (BZW1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19917-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 19917-1-AP (BZW1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19917-1-AP (BZW1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 19917-1-AP (BZW1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung cancer using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 19917-1-AP (BZW1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 19917-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 19917-1-AP (BZW1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 19917-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using BZW1 antibody (19917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: MCF7 cells, HeLa cells
• Positive IHC detected in: human testis tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

19917-1-AP targets BZW1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: BZW1 fusion protein Ag13830
• Full Name: basic leucine zipper and W2 domains 1
• Calculated Molecular Weight: 353 aa, 41 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC001804
• Gene Symbol: BZW1
• Gene ID (NCBI): 9689
• RRID: AB_10693530
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IF	Leukemia; IPO11 regulates the nuclear import of BZW1/2 and is necessary for AML cells and stem cells.; Authors - Boaz Nachmias
human,mouse	WB,IHC,IF	Genomics; BZW1 as an oncogene is associated with patient prognosis and the immune microenvironment in glioma; Authors - Linyao Zhao ; KD Validated