Beta-2-Microglobulin Polyclonal antibody

Beta-2-Microglobulin Polyclonal Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 30226-1-AP

Synonyms

B2M, beta 2 microglobulin, HDCMA22P

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Validation Data Gallery

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  WB analysis using 30226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 30226-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 30226-1-AP (Beta-2-Microglobulin antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IF Staining of NCCIT using 30226-1-AP

  Immunofluorescent analysis of (4% PFA) fixed NCCIT cells using Beta-2-Microglobulin antibody (30226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, U-937 cells, Jurkat cells, Raji cells
• Positive IF detected in: NCCIT cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30226-1-AP targets Beta-2-Microglobulin in WB, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: fusion protein
• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• Observed Molecular Weight: 12-14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions as a result of shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.