Beta-2-Microglobulin Recombinant monoclonal antibody, PBS Only

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  WB analysis using 83683-8-RR (same clone as 83683-8-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83683-8-RR (Beta-2-Microglobulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83683-8-PBS in a different storage buffer formulation.

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  IF Staining of Jurkat using 83683-8-RR (same clone as 83683-8-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Beta-2-Microglobulin antibody (83683-8-RR, Clone: 241308F6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83683-8-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83683-8-RR

  Biolayer interferometry (BLl) kinetic assays of 83683-8-RR against Human Beta-2-Microglobulin were performed. The affinity constant is below 1 pM.

Beta-2-Microglobulin Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 83683-8-PBS

Clone No.241308F6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

B2M, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3, CDABP0092

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Product Information

83683-8-PBS targets Beta-2-Microglobulin in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2284

  Product name: Recombinant Human B2M protein (mFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-mFc

  Tag: C-mFc

  Domain: 21-119 aa of BC032589

  Sequence: IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• Observed Molecular Weight: 12 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• ENSEMBL Gene ID: ENSG00000166710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61769
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.