FITC Plus Anti-Human Beta-2-Microglobulin Rabbit Recombinant Antibody

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• FITC Plus Anti-Human Beta-2-Microglobulin Rabbit Recombinant Antibody (FITC-98165)

Beta-2-Microglobulin Uni-rAb^® Recombinant Antibody for FC

Cat No. FITC-98165

Clone No.241308D5

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

B2M, 241308D5, beta 2 microglobulin, beta 2-Microglobulin, Beta-2-microglobulin form pI 5.3

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  FC experiment of human PBMCs using FITC-98165

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human Beta-2-Microglobulin Rabbit RecAb (FITC-98165, Clone: 241308D5) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using FITC-98165

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human Beta-2-Microglobulin Rabbit RecAb (FITC-98165, Clone: 241308D5) (red) or FITC Plus Rabbit IgG Isotype Control Recombinant Antibody (FITC-98136, Clone: 240953C9) (blue). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed.

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 5 ul per 10^6 cells in a 100 µl suspension

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.

Sample-dependent, Check data in validation data gallery.

Product Information

FITC-98165 targets Beta-2-Microglobulin in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2284

  Product name: Recombinant Human Beta-2-Microglobulin protein (mFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-mFc

  Tag: C-mFc

  Domain: 21-119 aa of BC032589

  Sequence: IQRTPKIQVYSRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM

• Full Name: beta-2-microglobulin
• Calculated Molecular Weight: 119 aa, 14 kDa
• GenBank Accession Number: BC032589
• Gene Symbol: B2M
• Gene ID (NCBI): 567
• ENSEMBL Gene ID: ENSG00000166710
• Conjugate: FITC Plus Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 495 nm / 524 nm
• Excitation Laser: Blue laser (488 nm)
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P61769
• Storage Buffer: PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3.
• Storage Conditions: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Background Information

Beta-2-microglobulin (B2M) is a component of MHC class I molecules, which are present on the surface of nearly all nucleated cells. It can be found in body fluids under physiologic conditions due to shedding from cell surfaces or intracellular release. B2M has various biological functions, including antigen presentation. Investigations reveal that increased synthesis and release of B2M are present in several malignant diseases.